摘要
烟青虫属鳞翅目（Ｌｅｐｉｄｏｐｔｅｒａ）昆虫。前人研究表明，苏云金杆菌（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ）中的Ｂｔ毒蛋白对其具有很强的毒杀作用。设法将Ｂｔ基因导入到烟草中，是防治这类虫害的一种有效途径。８０年代后期以来，为了提高Ｂｔ基因在植物...
Transformation of the leave of tobacco cultivar samson with plasmids pKUB、pKUC & pKSB carrying codon optimized Bt genes( cryIA(b) and cryIA(c) )was carried out using on Agrobacterium mediated method.A total of 27 R 0 regenerated plants were obtained.Integration of the Bt genes into tobacco genome was confirmed by southern hybridization and Western analysis.Assays for leaf GUS activity of R 1 lines were conducted. Of 21 lines tested,19 were GUS positive,one was negative,one segregating.The progenies of R 1 plants with homozygous gus genes were GUS positive as well.The PCR assays for R 1 and R 2 plants displayed the expected amplified bands.Upon feeding the tabacco budworm with leave from R 1 plants,the pathological changes occurred in the midgut of the insect were observed.The cross section of midgut of the poisoned worm in comparison with the healthy one clearly shows that the inner stratum of midgut have been completely broken down or striped off the outer structure.The content of Bt toxic protein in transgenic tabacco leave measured with Western dot blot method was above 0.2% on an average.
出处
《生物工程学报》
CAS
CSCD
北大核心
1999年第3期388-392,共5页
Chinese Journal of Biotechnology
关键词
转基因烟草
BT基因
农杆菌
介导法
烟青虫
Transgenic tobacco, Bt gene, cryIA(b) and cryIA(c) ,agrobacterinm mediated method
