摘要
目的:了解视网膜色素上皮(retinal pigment epithelium,RPE)细胞核仁组成区相关嗜银蛋白(ar-gyrophilic nucleolar organiser region-associated proteins,AgNORs)的表达量及柔红霉素对其影响。方法:将柔红霉素加入培养人RPE细胞的培养液中(终浓度为180μg/L)作用12小时,撤药后继续培养24小时。对培养细胞行AgNORs染色,图像分析染色结果。结果:对照组和柔红霉素组AgNORs面积(μm^2/核)分别为41.61±4.52和13.66±0.65(P<0.01);平均面密度(AgNORs面积与胞核面积之比)(%)为20.37±1.97和13.06±2.44(P<0.01)。结论:柔红霉素抑制体外培养人RPE细胞核仁组成区转录活性可能是其细胞毒作用的机理之一。眼科学报1999;15:70—73。
Purpose : To investigate the effect of daunoblastina on argyrophilic nucleolar organiser region-associated proteins (AgNORs) in cultured human retinal pigment epithelium (RPE) cells.Methods:The culture medium of secondary cultured human RPE cells were replaced with a daunoblastina-containing one at a dose of 180μg/L,and the cells were incubated for 12 hr at 37℃. After that, the medium was withdrawn, fresh medium was added and incubation was carried out for an additional 24 hr. AgNORs were studied by using a silver staining technique and image analysis system.Results; The area of intranuclear AgNORs (μm2 /nucleus) in the control and the daunoblastina-treated group were 41.61±4. 52 and 13. 66 ± 0. 65 ( P <0.01), and the area density of intranuclear AgNORs(AgNORs/nucleus area ratio) ( % ) in the two groups were 20.37±1.97 and 13.06±2.44( P <0.01) respectively. Conclusion: Inhibiting nucleolar organiser region transcriptional activity may be partly responsible for the cytotoxicity of daunoblastina on the cultured human RPE cells. Eye Science 1999; 15: 70 - 73.
出处
《眼科学报》
1999年第2期70-73,共4页
Eye Science
基金
国家自然科学基金资助
基金号39700154
关键词
视网膜色素上皮
柔红霉素
核仁相成区
嗜银蛋白
retinal pigment epithelium
cell culture
daunoblastina
argyrophilic nu- cleolar organiser region-associated proteins
image analysis
