摘要
目的:从碱蓬中提取出水溶性粗多糖SP,经分离纯化得到SPB,研究SPB的纯度,性质和体外抗氧化能力。方法:粗多糖经DEAE Sepharose CL-6B柱层析分离纯化得到SPB,经HPLC法检验纯度,超氧阴离子清除实验和羟自由基清除实验等鉴定其抗氧化能力。结果:SPB是均一组分多糖,分子量在40万。结论:SPB有高于Vc的超氧阴离子清除作用,以及相当于Vc的羟自由基清除作用。
A water-soluble crude polysaccharide SP was obtained from Suaede Spp.After crude polysaccharide was fractionated and purified by DEAE Sepharose CL-6B column chromatography,SPB was obtained.The homogeneity was proved by HPLC method.SPB were homogeneous and.On the basis of antioxidant activities assay,SPB has stronger inhibitory activities for superoxide radical than Vc.
出处
《食品科技》
CAS
北大核心
2010年第9期236-238,共3页
Food Science and Technology
基金
华侨大学科研基金资助项目(08X0212)
国务院侨办基金资助项目(07QZR07)
关键词
碱蓬
纯化
抗氧化活性
Suaede Spp
purification
antioxidant activities
