摘要
目的探讨以人自体骨髓基质干细胞(hBMSC)作为种子细胞的组织工程化骨在治疗肿瘤术后下颌骨缺损的可行性。方法回顾性分析2005年1月至2005年12月收治3例组织工程骨修复颌面部肿瘤术后下颌骨缺损的临床资料。抽取人骼前上棘骨髓,分离出hBMSC,经体外诱导和扩增,将hBMSC与部分脱钙骨(30%～40%)复合,于体外培养1个月后,手术植入骨缺损区。分别于术后2周、3个月、2年进行PET-CT检查随访。结果 PET-CT示,术后3个月能形成组织工程化骨,并修复了骨组织缺损,临床治疗效果稳定。结论以自体hBMSC为种子细胞,利用组织工程技术可在人体内形成稳定的组织工程化骨组织,并能修复下颌骨缺损。
Objective To explore the feasibility of hBMSCs acting as seeding cell for tissue engineered bone in the repairing of mandibular after tumor surgery.Methods Three patients with mandibular defect were treated by tissue engineered bone after tumor surgery.The hBMSC were isolated by percolling gradient centrifugation from patient＇s bone marrow aspirated from iliac crest.The hBMSC were cultured in vitro and induced.The osteogenic induced hBMSC were in partly demineralized allogenic bone scaffold and cultured for 1 month.The defects were repaired with the cell-scaffold construct.All cases were followed up for half a month,3 months,2 years postoperatively by PET-CT.Results PET-CT demonstrated that tissue engineered bone was formed in 3 months postoperatively.The tissue engineered bone can repair bone defect,and maintained stable effect.Conclusion The tissue engineered bone from hBMSCS can be formed in human body stably and used for repairing mandibular defect with satisfactory results.
出处
《组织工程与重建外科杂志》
2010年第4期183-187,共5页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
骨髓基质干细胞
下颌骨缺损
组织工程骨
修复
Bone marrow stromal cell（BMSC）
Mandibular defect
Tissue engineered bone
Repair
