摘要
以不同浓度的硫鱼精蛋白（ＰＳ）对Ｖｅｒｏ细胞制备的Ⅰ，Ⅱ，Ⅲ型脊髓灰质炎口服活疫苗（ＯＰＶ）病毒悬液进行后处理去除细胞基质ＤＮＡ研究．ＰＳ浓度大于１ｍｇ／ｍＬ，纯化后疫苗中ＤＮＡ残余含量低于１００ｐｇ／剂量．随着病毒液中ＰＳ浓度的增加，ＤＮＡ残余含量减少，但病毒回收率亦降低．结果表明ＰＳ沉淀法，病毒回收率高，ＤＮＡ残余含量能达到ＷＨＯ规程ＤＮＡ限量要求，对脊灰病毒ｒｃｔ／４０特征及病毒壳蛋白等生物学性状无显著影响。
The effect of different concentrations of PS on the removal of cellular DNA from types Ⅰ, Ⅱ and Ⅲ OPV produced on Vero cells was examined.It was found that 1?mg/mL PS was able to remove residual cell DNA to meet the WHO requirements (content of DNA <100?pg/dose) and when PS concentrati on was increased,the DNA content and virus recovery rate decreased.It also show ed that the treatment of OPV with PS has no influence on rct/40 character and vi ral capsid.We demonstrated that precipitation of OPV with PS is simple method f or eliminating residual cellular DNA in OPV preparation on Vero cell.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1999年第3期192-194,共3页
Journal of Yunnan University(Natural Sciences Edition)
