摘要
以知母分蘖为试材,对知母的一步法快速繁殖体系进行了初步研究。结果表明,知母分蘖直接再生植株的最佳培养基是MS+KT 1 mg/L+NAA 0.5 mg/L;知母分蘖愈伤组织诱导和再生的最佳培养基是MS+KT 2 mg/L+NAA 0.5 mg/L。本实验构建了知母分蘖的一步法快速繁殖体系,为知母试管苗的工厂化生产提供了理论和技术基础。
One-step rapid propagation system of Anemarrhena asphodeloides was studied with its fillers as experimental material. The results showed that the best direct regeneration medium of A. asphodeloides filler was MS ＋ KT 1 mg/L ＋ NAA0.5 mg/L, and the best callus induction and regeneration medium ofA. asphodeloides tiller was MS ＋ KT 2 mg/L ＋ NAA 0.5 mg/L. The one-step rapid propagation system ofA. asphodeloides filler was built in this experiment, which would provide theoretical and technical basis for the factory production ofA. asphodeloides plantlets.
出处
《亚热带植物科学》
2010年第3期27-29,共3页
Subtropical Plant Science
基金
2009年江西省高校省级教改项目(JXJG-09-16-10)资助
关键词
知母
分蘖
愈伤组织
一步法快繁体系
Anemarrhena asphodeloides
tillers
callus
one-step rapid propagation system
