摘要
目的明确胆管癌细胞是否存在胆囊收缩素（ｃｈｏｌｅｃｙｓｔｏｋｉｎｉｎ，ＣＣＫ）、胃泌素（ｇａｓｔｒｉｎ）自分泌机制。方法采用免疫组织化学及放射性免疫分析技术，探测了胆管癌细胞ＱＢ、ＯＣ及４２例胆管癌标本中ＣＣＫ８和Ｇａｓｔｒｉｎ１７的表达，胆管癌细胞ＱＢ、ＯＣ培养上清及细胞提取液中ＣＣＫ８和Ｇａｓｔｒｉｎ１７的含量，胆管癌患者手术前后空腹血浆ＣＣＫ８和Ｇａｓｔｒｉｎ１７浓度的改变。结果发现胆管癌细胞ＱＢ和ＯＣ及胆管癌标本中均无ＣＣＫ８和Ｇａｓｔｒｉｎ１７的表达；ＱＢ和ＯＣ中未检测到ＣＣＫ８和Ｇａｓｔｒｉｎ１７的存在；胆管癌患者手术前后空腹血浆胆囊收缩素、胃泌素含量无明显变化。结论胆管癌细胞无自分泌胆囊收缩素、胃泌素现象存在。
Objective To identify the
autocrine of CCK and gastrin in cholangiocarcinoma cells. Methods The expression of CCK 8
and gastrin 17 and concentration of CCK 8 and gastrin 17 in cholangiocarcinoma cell lines QB
and OC and in samples of 42 patients with cholangiocarcimoma were detected by
immunohistochemistry (IH) and radioimmunoassay (RIA). Results No expression of CCK and
gastrin had been identified in QB and OC cells, nor in samples of cholangiocarcinoma. The
study by demonstrated that no significant change of serum level of CCK and gastrin were found
before and after operation or vs control group; nor CCK and gastrin were detected in QB and OC
cells. Conclusion It is shown that no autocrine of CCK and gastrin existed in cholangicarcinoma
cells.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第2期117-119,共3页
Chinese Journal of Digestion
