摘要
目的探讨β 溶血型链球菌所诱发的急性点滴型银屑病的发病机制。方法应用MTT方法观察急性点滴型银屑病患者外周血淋巴细胞(PBMC)对链球菌抗原的反应 ,用 3H TdR掺入法和免疫组织化学方法检测链球菌抗原刺激的PBMC培养上清液对人类角质形成细胞株COLO 16DNA合成以及HLA DR、ICAM 1分子表达的影响。结果急性点滴型银屑病患者PBMC对链球菌抗原的反应较对照组更为明显(P<0.01) ,其24h链球菌抗原刺激的PBMC培养上清液对角质形成细胞的促增殖作用较对照组显著增强(P<0.01) ,并能诱导角质形成细胞表达HLA DR和ICAM 1。结论具有银屑病素质的个体体内可能存在链球菌抗原特异性的T淋巴细胞 ,受链球菌抗原或超抗原作用后增殖 ,产生大量细胞因子 ,导致角质形成细胞过度增生和HLA DR、ICAM 1异常表达。
ObjectiveToinvestigatethe pathogenesisofacuteguttatetype psoriasis(AGP)in ducedbystreptococcalinfection.MethodsThe proliferativeresponsesofperipheralbloodmononuclear cells(PBMCs)tostreptococcalantigensweredetectedbyusingMTTmethods.Effectsofsupernatantsof PBMCsculturedwithstreptococcalantigensonDNAsynthesisofkeratinocytesweremeasuredby3H TdR incorporationassay,andimmunohistochemicalstainingwasusedtoexamineHLA DRandintercellularadhe sionmolecule1(ICAM 1)expressiononkeratinocytes.ResultsTheproliferativeresponsesofPBMCsto streptococcalantigensweregreatlyenhancedinpatientswithAGPthanthoseincontrols(P<0.01).The supernatantsofPBMCsin patientswithAGPstimulatedbystreptococcalantigenscouldsignificantlyin crease3H TdRincorporationwithkeratinocytes(P<0.01).Andthesesupernatantsfrompatientswith AGPcouldalsoinducetheexpressionofHLA DRandICAM 1inhumankeratinocytes.Conclusion Theremightbestreptococcalantigen specificTlymphocytesinAGPpatients.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1999年第2期99-100,共2页
Chinese Journal of Dermatology
