摘要
以愈伤组织再生体系的4种愈伤组织和气生根再生体系的再生团块为材料,测定它们的超氧化物含量、保护酶活性和丙二醛含量等相关衰老生理指标.结果显示：超氧化物含量、超氧化物歧化酶（SOD）活性及过氧化氢酶（CAT）活性均为黄绿愈伤组织最高,金黄愈伤组织和深绿愈伤组织居中、再生团块和褐色愈伤组织较低;总谷胱甘肽过氧化物酶（GPX）活性以褐色愈伤组织最高,深绿、金黄和黄绿愈伤组织次之,再生团块最低;丙二醛（MDA）含量以金黄愈伤组织最高,褐色、黄绿和深绿愈伤组织次之,再生团块最低.研究表明,再生团块的保护酶活性、超氧化物含量和MDA含量均比4种愈伤组织低,其细胞膜脂过氧化程度最低,而抗氧损伤的能力更强;再生团块的抗衰老能力最强,其次为分化能力较强的黄绿愈伤组织,而分化能力较差的深绿愈伤组织、金黄愈伤组织和褐色愈伤组织的抗衰老能力较弱;利用气生根再生团块建立的快繁体系比用愈伤组织建立的快繁体系分化能力强、不易衰老,更具优越性.
The senescent index between four calli originated from leaves and RMBs（regeneration mass blocks） from aerial roots were compared.The results showed that the orders by the values of superoxide,the activities of SOD（superoxide dismutase） and CAT（catalase） were yellow green callus,golden callus,dark green callus,RMB and brown callus.The highest activities of GPX（glutathione peroxidase） were showed in brown callus,followed by dark green,yellow green callus,golden callus and RMB.For MDA（malondialdehyde）,the highest contents were observed in golden callus,followed by brown callus,yellow green callus,dark green callus and RMB.It was demonstrated that the activities of SOD and CAT had synergistic effects in eliminating oxidative stress,and the higher activities of SOD and CAT in RMBs suggested their greater ability of eliminating oxidative stress.The enzyme activities of GPX in RMB were significantly lower than that in other calli.In other hand the activities of GPX were relatively lower in yellow green callus,the lower contents of super-oxides and MDA might be the results of higher antioxidant activities.In short rapid propagation of RMBs had advantages over that of other calli because of their higher anti-oxidant activities.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第8期1639-1645,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
河北省科技厅生物工程项目(04547008D-2)
唐山市生物与化学重点实验室项目(04360701B-51)
关键词
红掌
再生体系
衰老
生理指标
Anthurium andraeanum
regeneration system
aging
physiological index
