摘要
目的:研究表明miRNAs在人类恶性肿瘤的发生发展过程中起着重要作用,本研究检测6种miRNAs:miR-98、let-7d、let-7g、miR-96、miR-182及miR-183在前列腺癌组织中的表达情况及其临床意义。方法:采用原位分子杂交方法,结合组织芯片技术分别检测38例BPH和52例前列腺癌中6种miRNAs的表达情况。并对前列腺癌组织中6种miRNA与Gleason评分,临床分期及6种miRNA间进行相关分析。结果:6种miRNAs中,与BPH组织相比,在前列腺癌组织中表达降低的为miR-98、let-7d、let-7g;而表达升高的为miR-96、miR-182及miR-183,差异均有统计学意义(P<0.05)。6种miRNAs的表达与前列腺癌的Gleason评分均相关(P<0.05),但与患者的年龄及血清PSA水平均无明显相关性(P>0.05)。其中miR-96和miR-182的表达与前列腺癌的临床分期均有相关性(P<0.05),miR-98和miR-96的表达均与肿瘤累及前列腺的叶数存在相关性(P<0.05)。另外,前列腺癌组织中,miR-96、miR-182及miR-183的表达彼此之间呈正相关(P<0.01,r分别为0.41,0.44),let-7d与let-7g的表达之间呈正相关(P<0.01,r=0.46),miR-98与let-7d及let-7g的表达之间呈正相关(P<0.05,r分别为0.31,0.34)。结论:miR-98、let-7d、let-7g、miR-96、miR-182及miR-183构成的miRNA表达谱在一定程度上反映了前列腺癌的生物学行为,可能作为前列腺癌早期诊断和预后评估的重要生物标记物。
Objective： Experimental evidence shows that microRNAs play an important role in the initiation and progression of human malignancies. The present study aimed to investigate the expressions of 6 microRNAs in prostate cancer （PCa） and their clinical significance. Methods： We investigated the expression profiles of 6 microRNAs （let-7g, let-7d, miR-98, miR-96, miR-182 and miR-183 ） using the method of locked nucleic acid （LNA）-modified oligonueleotide in situ hybridization （ISH） and the technology of tissue mieroarray （TMA） with the formalin-fixed paraffin-embedded （FFPE） specimens from 52 patients with PCa and 38 with benign prostatic hyperplasia （BPH）. Then we analyzed the correlation among the expressions of the 6 microRNAs in PCa and their correlation with the Gleason score and clinical stages of PCa. Results ： Compared with BPH, the PCa patients showed decreased expressions of miR-98, let-7d and let-7g, and decreased expressions of miR-96, miR-182 and miR-183, with statistically significant differences between the two groups （ P 〈 0.05 ）. The positive rate of the 6 microRNAs was significantly correlated with the Gleason grades of PCa （P 〈0.05） , but not with the age and serum PSA concentration of the patients （P 〉0.05）. The expressions of miR-96 and miR-182 were correlated with the clinical stages of the tumor （ P 〈 0.05 ）. There was a positive correlation among the expressions of miR-96, miR-182 and miR-183 （ P = 0.00, r = 0.41 ）, as well as between the expressions of let-7d and let-7g （ P = 0.00, r = 0.46） in the PCa tissues. And the expression of miR-98 was positively correlated with those of let-7d and let-7g （P = 0.00, r = 0.46）. Conclusion： The expression profiles of the microRNAs let-7d, let-7g, miR-98, miR-96, miR-182 and miR-183 reflect the biological behavior of PCa to some extent, and might be important biomarkers for the early detection and prognostic assessment of prostate cancer. Nat/ J Androl, 2010, 16 （7）： 599-605
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第7期599-605,共7页
National Journal of Andrology
