摘要
目的分析奶牛胎衣不下(Retained fetal membranes,RFM)与胎儿胎盘中诱导型一氧化氮合酶(Induction nitric oxid esynthase,iNOS)、内皮型一氧化氮合酶(Endothelial nitric oxide synthase,eNOS)和基质金属蛋白酶-2(Matrix metallopeptidase-2,MMP-2)表达的相关性,探讨RFM发生的分子机制。方法 RT-PCR扩增、克隆产后胎衣正常脱落(No retention of fetal membranes,NRFM)和RFM奶牛胎儿胎盘中iNOS、eNOS和MMP-2基因,以牛β-actin基因为内参,采用荧光定量PCR法检测RFM和NRFM奶牛胎儿胎盘组织中iNOS、eNOS和MMP-2基因表达的差异。结果克隆的iNOS、eNOS和MMP-2基因片段与GenBank中登录的核苷酸序列同源性均达100%。RFM组iNOS基因mRNA的转录水平明显高于NRFM组(P<0.05),eNOS和MMP-2基因mRNA的转录水平则明显低于NRFM组(P<0.05)。结论 iNOS、eNOS和MMP-2基因mRNA的转录水平与RFM的发生密切相关。
Objective To analyze the retained retention of fetal membrane（RFM） of cows to the expressions of induction nitric oxide synthase （iNOS）, endothelial nitric oxide synthase （eNOS）and matrix metallopeptidase-2 （MMP-2）in placenta as well as the molecular mechanism of onset of RFM. Methods The iNOS, eNOS and MMP-2 genes were amplified by RT-PCR from the placentas of cows with no RFM （NRFM）and with RFM separately and cloned, using bovine β-actin as an internal reference, and the expression levels were determined by fluorescent quantitative PCR. Results The iNOS, eNOS and MMP-2 genes were cloned, of which the homologies of nucleotide sequences were 100% to those reported in GenBank. The transcription level of iNOS mRNA in the placenta of cows in RFM group was significantly higher, while those of eNOS and MMP-2 mRNAs were significantly lower than those in NRFM group（P 0. 05）. Conclusion The transcription levels of iNOS, eNOS and MMP-2 mRNAs were closely related to the onset of RFM.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第8期825-828,838,共5页
Chinese Journal of Biologicals
基金
黑龙江省青年科学技术专项资金项目(QC06C001)
关键词
胎衣不下
一氧化氮合酶
基质金属蛋白酶2
荧光定量PCR
Retained fetal membrane（RFM）
Nitric oxide synthase（NOS）
Matrix metallopeptidase 2
Fluorescent quantitative PCR
