摘要
目的：ｓＨＬＡⅠ可预测器官移植排斥反应及监控某些疾病。为了介绍ｓＨＬＡⅠ类抗原定量检测技术及中国人ｓＨＬＡⅠ正常值。方法：采用ＥＬＩＳＡ双抗夹心法，以Ｗ６／３２ｍＡｂ包被酶标板，加被检血清，再加β２ｍＨＲＰ及底物显色。以不同浓度ｓＨＬＡⅠ标准品的结果绘出标准曲线并测定１１３例正常人ｓＨＬＡⅠ含量。结果：ＥＬＩＳＡ法可以准确定量测定血清中ｓＨＬＡⅠ，中国人ｓＨＬＡⅠ正常值为７８９２７±５０６３２ｎｇ／ｍｌ。结论：ＥＬＩＳＡ法检测ｓＨＬＡⅠ具有特异、敏感及重复性。
Objective:sHLA Ⅰ antigens may predict the onset of rejection after organ transplantation and monitor some diseases. The method of quantification of sHLA Ⅰ antigens and its normal level in Chinese were reported. Methods:Sandwich ELISA assay was used. After microtiter plate coated by W6/32 mAb, serum was added, followed by incubation with anti β 2m HRP and the color development. Standard curve was obtained by quantification of sHLA Ⅰstandard reagent in series dilution. The amount of sHLA Ⅰantigens was examined in 113 normal healthy volunteers. Results: sHLA Ⅰ antigens can be exactly quantificated by sandwich ELISA assay. The level of sHLA Ⅰ antigens in normal individual is 789.27±506.32 ng/ml. Conclusion: Sandwich ELISA assay is special, sensitive and reproducible for sHLA Ⅰ antigens, and the results can be compared each other only when reagents and protocols are the same.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第5期228-229,共2页
Chinese Journal of Immunology
关键词
sHLA
ELISA
定量检测
移植免疫学
Soluble human leukocyte antigens(sHLA) Enzyme linked immunosorbent assay(ELISA) Quantification
