摘要
目的:对19株阪崎肠杆菌分离株和1株阪崎肠杆菌标准菌株进行脉冲场电泳(PFGE)分型分析。方法:利用限制性内切酶Xba1对阪崎肠杆菌基因组DNA进行酶切和PFGE分析,并利用BioNumerics软件进行聚类分析。结果:20株阪崎肠杆菌PFGE带型表现出多样性,在可分成16种PFGE型。BQ9、BQ11、BQ12、BQ13和BQ14相似性系数为100%,属于同一PFGE型别。BQ9、BQ11、BQ13和BQ14是同一生产厂家不同品牌的四种产品。其余各分离株和标准菌株的酶切图谱各不相同,相似性系数均在90%以下,被确定为不同PFGE型。结论:PFGE酶切带型与菌株来源有相关性,可应用于阪崎肠杆菌的分子分型和溯源。
Objective：To analyze the fingerprint of Enterobacter sakazakii,nineteen isolates and one type strains were analyzed with the pulsed-field gel electrophoreses（PFGE）.Methods： All of strains were digested with restriction endonueleases XbaI respectively,and the PFGE patterns were analyzed with BioNumerics software.Results： PFGE with restriction endonucleases XbaI gave 16 kinds of PFGE patterns respectively,twenty strains of Enterobacter sakazakii gave different patterns except BQ9,BQ11,BQ12,BQ13 and BQ14,showed the same PFGE pattern.There is no significant correlation between PFGE patterns and source information of corresponding samples except BQ9,BQ11,BQ13 and BQ14,which were isolated from the infant formula with same professional manufacturer and same brand but different goods.Conclusion： PFGE restriction band pattern was correlated with the strain of origin.The above PFGE methods can be used to the molecular typing and tracing of Enterobacter sakazakii.
出处
《中国卫生检验杂志》
CAS
2010年第9期2126-2127,共2页
Chinese Journal of Health Laboratory Technology
关键词
阪崎肠杆菌
脉冲场凝胶电泳
分子分型
Enterobacter sakazakii
Pulsed-field gel electrophoreses
Molecular typing
