摘要
采用细胞分裂抑制剂阿糖胞苷(Cytosine Arabinoside,Ara-C)抑制体外培养小鼠小脑组织中少突胶质细胞的增殖而造成小脑组织的脱髓鞘模型,以10μg/ml阿糖胞苷处理7天为最佳剂量。利用该模型与大鼠视神经组织联合培养2周后,少突胶质细胞自视神经迁至经Ara-C处理的小脑组织中,并在视神经附近形成髓鞘,表明异种动物之间神经组织联合培养能形成髓鞘。少突胶质细胞在形成髓鞘前先进行分裂增殖。本实验建立和采用的体外小脑组织脱髓鞘模型和联合培养系统对研究影响中枢神经髓鞘再生的因素是有效的。
The demyelinated mouse cerebellar explants were obtained successfully through prohibiting the division of oligodendrocytes by Cytosine Arabinoside (Ara-C), a DNA synthesis inhibitor. Among all the different dosages of Ara-C in media screened, we found that 10μg/ml Ara-C for 7 d. i. v. was satisfactory to our ordinary use. After 2 week co-culture of Ara-C treated mouse cerebellum and rat optic nerve, it was found that normal oligodendrocytes migrated out of the xenograft optic nerve into the demyelinated cerebellar explant and formed myelin sheath near the optic nerve, indicating that xenograft co-culture of nerve tissues is possible for myelination. The present experiment also indicated that oligodendrocytes went mitosis before myelination in our co-culture system. The demyelinated model in vitro and the co-culture system of the present experiment might be useful in the studies of myelination and remyelination in the central nervous system.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
1990年第1期29-32,共4页
Chinese Journal of Neuroanatomy
关键词
脱髓鞘模型
组织培养
小脑
视神经
demyelinated model, tissue culture, oligodendrocyte, remyelination
