摘要
用芍药科植物牡丹根皮为材料，采用三种不同的方法提取，经ＤＥＡＥ－纤维素ＤＥ－５２离子交换柱纯化，获得丹皮多糖（ＰＳＭ）纯品。动物试验证明，三种不同方法提取ＰＳＭ的纯品，在降血糖活性上有显著差异。蒸馏水浸提得到的纯品ＰＳＭ２ｂ降低血糖率为５９．９％（Ｐ＜０．０１，５０ｍｇ／ｋｇ）和５３．１％（Ｐ＜０．０１，１００ｍｇ／ｋｇ）。温水提取的纯品ＰＳＭ３ｂ降低血糖率为５５．８％（Ｐ＜０．０１，５０ｍｇ／ｋｇ体重）和４５．９％（Ｐ＜０．０５，１００ｍｇ／ｋｇ体重），沸水提取的纯品ＰＳＭ４Ｃ无降血糖活性。
Mudan cortex of paeonia
suffrulicosa was extracted with water ( 20-30℃), hot water (40℃) and boiling water (100℃),
treated with ethanol to remove and obtain the crude polysaccharide. Then crude PSM were
purified by DEAE Cellulose DE-52 column to get pure PSM. The laters were given to mice
intraperitoneally. Results showed that PSM extracted with water (20-30℃) and hot water (40℃)
can markedly lower the serum glucose, but PSM extracted with boiling water (100℃) had no
hypoglycemic activity.
出处
《安徽大学学报（自然科学版）》
CAS
1999年第2期90-93,共4页
Journal of Anhui University(Natural Science Edition)
基金
安徽省教委重点资助项目
