摘要
采用传统的平板计数法和变性梯度凝胶电泳技术,对转chi＋rip（几丁质酶和核糖体失活蛋白）双价抗真菌基因大豆转基因G0431以及其亲本非转基因大豆黑农35种植后的根部土壤可培养细菌（含芽胞杆菌、产荧光假单胞菌）、真菌、放线菌进行平板计数及细菌和真菌的群落结构分析.结果表明,二者根部土壤可培养细菌（含芽胞杆菌和产荧光假单胞）、真菌和放线菌的数量并无显著差异;此外,变性梯度凝胶电泳图谱的聚类分析结果显示,时间变化是造成根部土壤细菌和真菌群落结构变化的主要因素,而同一时间内转基因G0431和黑农35根部土壤的细菌和真菌群落结构之间并无显著不同.在大豆的整个生长期内,根部土壤细菌群落多样性指数为5.32～5.37,群落分布均匀度指数为0.91~0.95;根部土壤真菌群落多样性指数为4.78～4.91,群落分布均匀度指数为0.81～0.89.以上结果说明,几丁质酶和核糖体失活蛋白双价基因的导入并没有对大豆根际可培养细菌（含芽胞杆菌和产荧光假单胞）、真菌和放线菌的数量以及细菌和真菌的群落结构产生显著影响.
G0431 is the genetically modif ied soybean by transformation of two anti-fungus genes,＂chi＂（chitinase gene from bean） and ＂rip＂（barley ribosome-inactivating protein gene） into the natural strain,Heinong 35.The methods of classical plate counting and denaturing gradient gel electrophoresis（DGGE） were employed to analyze the amounts of culture microbes in rhizosphere soil and the community structure of bacteria（including Bacillus and fluorescent Pseudomonas）,fungi and actinomycetes.The results showed that there was no signif icant difference between the amounts of culture soil microorganisms in the rhizosphere soil of the transgenic G0431 and Heinong 35.Moreover,the results of cluster analysis of DGGE profile of bacteria and fungi indicated that the time was the main factor which affected the community structure of rhizosphere soil microbes.For the two investigations,the Shannon＇s index of the bacterial community in the rhizosphere soil was 5.32～5.37 and the evenness index was 0.91～0.95;the Shannon＇s index of the fungal community in the rhizosphere soil was 4.78～4.91 and the evenness index was 0.81～0.89.The same as the community structure of bacteria and fungi,there was no remarkable difference in the rhizosphere soil between the transgenic and non-transgenic soybean in the numbers of culture bacteria（including Bacillus and fluorescent Pseudomonas）,fungi and actinomycetes.It implied that the transformation of the two extra antifungus genes into soybean didn＇t cause any signif icant effect on the amounts and community structure of its rhizosphere soil microbes.Fig 4,Tab 2,Ref 22
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第4期509-514,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家重点基础研究发展项目("973"计划
No.2007CB109203)
高技术研究发展项目("863"计划
No.2008AA10Z153)
国家转基因生物新品种培育重大专项(No.2008ZX08011-003)资助~~
关键词
转基因大豆
变性梯度凝胶电泳
土壤微生物
群落结构
transgenic soybean
denaturing gradient gel electrophoresis
soil microorganism
community structure
