摘要
农杆菌介导是大豆遗传转化的主要手段。该文从受体基因型及外植体选择、菌株筛选及载体改造、标记基因选用和添加剂应用等方面,介绍了近年来对农杆菌介导大豆遗传转化方法所做的改进,分析了存在问题,展望了近期发展方向。
Genetic transformation of soybean（Glycine max（L） Merr.） depends mainly on Agrobacterium mediation system.In this review,the authors introduced the modifications of the technical procedures in this system including the selection of genotypes,explants,strains and makers,plasmid reconstruction,and application of additive agents,etc.We analyzed the disadvantages of this system and took an outlook for the improvement of Agrobacterium-mediated transformation system in soybean in the near future as well.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第4期696-701,共6页
Soybean Science
基金
转基因生物新品种培育重大专项资助项目(2008ZX08004-001,2008ZX08010-004-004)
农业部财政部现代农业产业技术体系建设专项资助项目(nycytx-004)
关键词
大豆
农杆菌介导
转基因
Soybean
Agrobacterium-mediated
Transformation
