摘要
利用Phytome、TAIR、NCBI网站及其数据库和MEGA 4.0、Clustal X软件,对大豆(Gly-cine max)基因组中TOC1同源基因进行了生物信息学分析,确定了GmTOC1基因及其在染色体上的物理分布、内含子-外显子结构,进行了氨基酸序列比对,并研究了其系统发育学地位。结果表明,GmTOC1与AtTOC1亲缘关系较近,都具有保守的调节区域,而且可能具有相似的生物钟调控功能。此外发现,大豆基因组中含有4个拷贝的TOC1同源基因,它们在生物进化过程中保留了与拟南芥同源的结构和功能。
Bioinformatics analysis about four copies of TOC1 homolog in soybean genome would provide foundation for its function research.Mining TOC1 homologs in soybean genome（named GmTOC1s）,analysis of chromosomal locus,intron-exon structure of genes,amino acid sequence alignment and phylogenetic relations were carried out by MEGA 4.0 and Clustal X software within Phytome,TAIR and NCBI website and their databases.This results showed that GmTOC1s and AtTOC had near relations and both of which have CCT domain and similar diurnal expressions in LD and SD of these genes.In addition,4 highly conserved TOC1 homologs copy in soybean genome were founded,which preserved similar construction and fouction to the gene in Arabidopsis.
出处
《河南农业科学》
CSCD
北大核心
2010年第8期22-27,32,共7页
Journal of Henan Agricultural Sciences
基金
国家863计划(2007AA10Z119
2006AA10A111)
关键词
大豆
TOC1
生物钟
开花
系统发育
Glycine max
TOC1
Circadian clock
Flowering
Phylogenesis
