摘要
目的探讨小鼠皮肤切创愈合过程中损伤区大麻素Ⅰ型受体(cannabinoid receptorⅠ,CB1R)的表达及不同时间的变化规律。方法应用免疫组织化学和Western印迹法检测小鼠皮肤切创后不同时间段CB1R的变化情况。结果正常组织中有少量CB1R的表达,位于表皮、毛囊、皮脂腺、皮肌层。伤后6～12 h,中性粒细胞不表达CB1R,伤后1～5 d,阳性染色以单核细胞和成纤维细胞为主。伤后7～14 d,CB1R阳性着色以成纤维细胞为主。阳性细胞率6h～3d逐渐升高,5d达到高峰,7～14d逐渐降低。经Western印迹法显示各个时间段均有CB1R的阳性条带,其中5d为CB1R表达的高峰。结论在皮肤损伤愈合过程中CB1R被激活,CB1R表达于单核细胞,可能参与炎症反应,CB1R在损伤周边区表达于成纤维细胞,可能参与皮肤损伤后的愈合,其变化规律可用于损伤时间的推断。
Objective To investigate the expression of cannabinoid receptor Ⅰ（CB1R） during mice skin incised wound healing course and time-dependent changes of CB1R in wound age determination.Methods The changes of CB1R expression in skin incised wound were detected by immunohistochemistry and Western blotting.Results The control group showed a low expression of CB1R detected mainly in epidermis,hair follicles,sebaceous gland and dermomuscular layer.CB1R expression was undetectable in neutrophils in the wound specimens from 6 h to 12 h post-injury.CB1R positive cells were mostly mononuclear cells（MNCs） and fibroblastic cells（FBCs） from 1 d to 5 d post-injury.CB1R positive cells were mostly FBCs from 7 d to 14 d post-injury.The ratio of the CB1R positive cells increased gradually in the wound specimens from 6 h to 3 d post-injury,reached peak level at 5 d,and then decreased gradually from 7 d to 14 d post-injury.The positive bands of CB1R were observed in all time points of the wound healing course by Western blotting.The expression peak showed at 5 d post-injury.Conclusion CB1R is activated during the wound healing course.The expression of CB1R is found in mononuclear cells,which could be involved in inflam-mation reaction.CB1R is observed in fibroblastic cells,which could participate in the wound healing.CB1R may be a potentially useful marker for determination of wound healing age.
出处
《法医学杂志》
CAS
CSCD
2010年第4期241-245,共5页
Journal of Forensic Medicine
基金
教育部高等学校博士学科点专项科研基金项目(200801590020)
