摘要
目的探讨梅毒酶联免疫吸附试验(ELISA)、梅毒甲苯胺红不加热血清反应素试验(TRUST)、梅毒胶体金试验(SYP)3种方法在梅毒诊断检测中的应用价值。方法用梅毒螺旋体明胶颗粒凝集试验(TPPA)确诊阳性的标本,根据不同滴度分别进行TRUST、ELISA、SYP检测,评价3种筛选方法的敏感性。结果 ELISA法的敏感性明显高于另外二种方法,但抗体滴度较低或过高时易出现假阴性。结论 ELISA法比SYP或TRUST法更适合用于梅毒临床筛查诊断。
Objective To study the diagnosis value of three common serological tests,enzyme-linked immunosorbent assay（ELISA）,toluidine red unheated serum reagin test（TRUST）,colloidal gold test for syphilis（SYP）.Method The sensitivity of three screening methods with TPPA-positive samples were evaluated according to its antibody titers of TPPA,respectively.Result The sensitivity of ELISA was significantly higher than the other two methods,but it was false-negative when the antibody titers are low or too high. Conclusion ELISA combinated with TRUST or SYP was more suitable for clinical screening and diagnosis.
出处
《南华大学学报（医学版）》
2010年第5期679-680,共2页
Journal of Nanhua University(Medical Edition)
