摘要
目的：为了解虎杖蒽醌化合物晶Ⅰ、晶Ⅳ抗ＣＶＢ３病毒的药效，从而明确虎杖的抗病毒性能并开发利用。方法：以病毒唑为阳性对照，利用空斑减数实验进行研究。结果：在ＨＥＰ２细胞系统中，晶Ⅰ对ＣＶＢ３直接杀灭、增殖抑制、感染阻断的ＥＤ５０分别为１．５４μｇ／ｍＬ、８．３４μｇ／ｍＬ、３．０６μｇ／ｍＬ，晶Ⅳ的相应系数分别为１．７９μｇ／ｍＬ、７．０５μｇ／ｍＬ、３．１７μｇ／ｍＬ，而病毒唑的相应系数分别为６．２６μｇ／ｍＬ、７．５５μｇ／ｍＬ、７．５０μｇ／ｍＬ。结论：晶Ⅰ对ＣＶＢ３的治疗指数（ＴＩ）为病毒唑对ＣＶＢ３的ＴＩ的４．７、１．０、２．９倍；晶Ⅳ对ＣＶＢ３的治疗指数（ＴＩ）为病毒唑对ＣＶＢ３的ＴＩ的２７．５、８．４、１８．８倍，提示晶Ⅰ、晶Ⅳ的抗ＣＶＢ３方面有一定优势。
:Objective:Rhizoma polygoni cuspidati is a chinese
medicinal herb with large antiviral range, in order to understand and exploit it's antiviral
properties, the authors extracted anthraquinones of it, that is cryatalline Ⅰ and crystalline Ⅳ,
and observed effects of anticoxsackie B 3 virus of them. Methods:Used ribavirin as positive
control, and by way of plaque reduction assays, carry out this research. Results:In HEP 2 cell
system, the ED 50 s of direct virucidal activity, the inhibition effects on the multiplication and
infection of coxsackie B 3 virus are 6.26μg/mL, 7.50μg/mL, 1.75μg/mL respectively, the
corresponding figures of crystalline Ⅰ are 1.54μg/mL, 8.34μg/mL, 3.06μg/mL separately,
and the corresponding figures of crystalline Ⅳ are 1.79μg/mL, 7.05μg/mL, 3.17μg/mL
repectively. Conclusion:The treatedment indices (TI) of crystalline Ⅰ to CVB 3 are 4.7, 1.0, 2.9
times of ribavirin TIs, the TIs of crystalline Ⅳ to CVB 3 are 27.5, 8.4, 18.8 times of ribavirin TIs,
they should be worth exploitation.
出处
《成都中医药大学学报》
1999年第2期41-43,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
湖北省自然科学基金
关键词
虎杖蒽醌化合物
柯萨基病毒
中医药疗法
实验
Anthraquinones of rhizoma polygoni cuspidati
coxsackie B 3 virus
pharmacology
