摘要
通过在Ｚａｒｒｏｕｋ培养基中加入不同浓度的Ｐｂ^（２＋）研究水环境污染对钝顶螺旋藻（Ｓｐｉｒｉｕｌｉｎａ ｐｌａｔｅｎｓｉｓ）生长、藻蓝蛋白和光合作用、膜脂过氧化反应及以超氧物歧化酶（ＳＯＤ）为代表的自由基清除系统的影响发现当 Ｐｂ^（２＋）浓度高于 ２０ｍｇ·Ｌ^（－１）时，钝顶螺旋藻生长受抑制，藻细胞随着浓度的升高和时间的延长而黄化死亡．细胞可溶性蛋白含量、藻蓝蛋白含量、超氧物歧化酶（ＳＯＤ）活性均随着Ｐｂ^（２＋）浓度增加而逐渐下降。是极显著的负相关．低浓度Ｐｂ^（２＋）使光合速率大幅度下降但完整细胞的吸收光谱在高浓度时才发生急剧变化．Ｐｂ^（２＋）处理使超氧阴离子^（Ｏ２－）和丙二醛（ ＭＤＡ）含量上升，其峰值分别出现在 ３０ｍｇ· Ｌ^（－１）和 ４０ｍｇ· Ｌ^（－１）处理组． ＳＯＤ活性在 ２０ｍｇ· Ｌ^（－１）以上显著下降，但其比活性在２０－５０ｍｇ·Ｌ^（－１）范围内仍维持较高水平，且相对稳定．
In this paper, we studied the growth of S. platensis in mimic Ph^(2+) polluted environment with different concentration from 10 to 80 mg' L^(-1). The results showed that the growth rate of S. platensis was inhibited by Ph^(2+) above 20mg' L^(-1) S. platensis yellowed and died with Ph'+ concentration increasing and time going. The solvable protein, phycocynin and the ratio of phycocynin to carotenonid were all decreased. Each had a noticeable correlation with the Ph^(2+) concentration. Lower concentration of Ph^(2+) could decrease the photosyntheitc O_2, evolution of S. platensis, yet the absorption spectrum of S. platensis was only seriously changed on high Ph^(2+) concentration. Contents of ^(O2-) and MDA were also changed, each reached its maximum at 30mg' L^(-1) and 40mg' L^(-1) The activity of SOD was decreased obviiusly when above 20mg L^(-l), but its relative activity was higher and quite steady at Ph'+ concentration from 20 to 50mg' L^(-1).
出处
《湖泊科学》
EI
CAS
CSCD
1999年第2期135-140,共6页
Journal of Lake Sciences
基金
江苏省自然科学基金!(BJ98077)
关键词
铅污染
钝顶螺旋藻
生长量
水环境污染
Ph^(2+) pollution
Spirulina platensis
growth rate
SOD activity
