摘要
目的：以大鼠肺Ⅱ型细胞为靶细胞，检测烹调油烟的细胞毒作用和对细胞ＤＮＡ的损伤。方法：烹调油烟颗粒提取物对大鼠肺Ⅱ型细胞染毒２４ｈ后，应用ＭＴＴ法、溴乙锭荧光法以及单细胞凝胶电泳技术检测烹调油烟对大鼠肺Ⅱ型细胞的细胞毒性和ＤＮＡ的损伤。结果：在受试剂量范围内，烹调油烟颗粒提取物具有细胞毒性，可致ＤＮＡ交联及断裂的作用随染毒剂量的增加而增强，并存在剂量－反应关系（ｒ细胞毒性＝－０．９４８，Ｐ＜０．０１；ｒ交联＝０．９７６，Ｐ＜０．０１；ｒ断裂＝０．９５７，Ｐ＜０．０１）。结论：烹调油烟颗粒提取物对大鼠肺Ⅱ型细胞具有细胞毒性作用和ＤＮＡ损伤作用，可使ＤＮＡ链间交联和ＤＮＡ单链断裂。
bjective: The purpose of the study was to detect the cytotoxicity and
DNA damage in rat pulmonary type II cells induced by cooking oil fume. Methods: Type II lung
cells were isolated from rat pulmonary tissues and after being cultured for 24 hours,they were
exposed to acetone extracts from vegetable oil fume(oil temperature:270).Cytotoxicity and DNA
interstrand crosslinks as well as DNA single strand breaks were detected by MTT assay,
ethidium bromide fluorescence assay and the single cell gel electrophoresis assay. Results:
The extracts had an obvious cytotoxicity and DNA damage effect to the cells and
doseresponsive relationships were found (rcytotoxicity=-0.948,P<0.01;rDNA
crosslinks=0.976,P<0.01;rDNA breaks=0.957,P<0.01). Conclusion: Cooking oil fume has a
cytotoxicity and genotoxicity to rat pulmonary cells, which may be a molecular basis for
carcinogenesis of cooking oil fume.
出处
《南京铁道医学院学报》
1999年第2期96-98,共3页
Journal of Nanjing Railway Medical College
基金
江苏省科委应用基础基金
