摘要
目的原肌球蛋白是肌球蛋白主要相关蛋白之一,在细胞骨架与运动中起着重要的作用。探讨原肌球蛋白在增生性瘢痕中的作用,有助于揭示瘢痕挛缩的产生机制。方法收集2006年3月-2008年7月48例患者不同时期增生与非增生瘢痕组织标本,利用基因芯片筛选出的瘢痕相关基因——原肌球蛋白基因特异片段,制备成寡核苷酸探针与瘢痕组织切片进行原位杂交。同时,将各标本进行原代细胞培养,制备成纤维细胞爬片,进行原位杂交。结果原肌球蛋白基因在3、6个月的增生性瘢痕中表达均明显强于9、12个月增生性瘢痕及非增生性瘢痕,阳性细胞比例也高于9、12个月增生性瘢痕及非增生性瘢痕。结论瘢痕增生挛缩与细胞骨架运动的相关基因存在密切关系,而原肌球蛋白可能在其中起着重要作用。
Objective Tropomyosin is one of the proteins of cytoskeleton and cell movement.The aim of this study is to investigate the effect of gene expression of fibroblast tropomyosin on the formation and contraction of hypertrophic scar.Methods According to the results of differently expressed genes,in hypertrophic scar by gene microarray,topomyosin,one of the most important genes,was selected and made into oligonucleotide probe.Twenty-four hypertrophic scars and 24 non-hypertrophic scars and 12 normal skins were used and these scar were taken on 3,6,9,and 12 months after burned between March 2006 and July 2008.Frozen section and cultured fibroblasts were made to detect the expression of the gene by in situ hybridization.Results Expression of tropomyosin was defected in scar tissue,but those in the hypertrophic scar on 3 and 6 months after burn were significantly stronger than those in the hypertrophic scar on 9 and 12 months after burn and non-hypertrophic scar.Conclusion Overexpression of cytoskeletal relative genes causes the contraction of scar and tropomyosin acts leading and key functions.
出处
《华西医学》
CAS
2010年第7期1206-1208,共3页
West China Medical Journal
基金
国家自然科学基金资助项目(30571922)
