摘要
用同位32~P标记乳酸脱氢酶-C(LDH-C)cDNA作为探针,与小鼠胸腺、脑、胰、心肌、骨骼肌、睾丸、肾、肺、肝的RNA以及人胰、皋丸、肝、骨骼肌、心肌及脑的RNA分别作点溃杂交(dot blot hybridization)及Northern印迹杂交,证实LDH-C基因只在睾丸中特异性表达。
By using a CsCl gradient and ultraoentrifngation on a email scale, RNA was extracted from a variety of human and mouse tissues, including the thymus, brain, spleen, pancreas, testis, kidney, lung, liver and cardiac and skeletal muscles separately. 32P-labelled, nick-translated mouse LDH-C (laotate denydrogenase-C) cDNA was used as a probe. Dot and Nothern blot hydridizabion was carried out. Eesults showed that the expression of LDH-C gene is tissue-specific, in other words. LDH-C gene is specifically only expressed in the testis.
出处
《上海医科大学学报》
CSCD
1990年第3期161-165,共5页
Journal of Fudan University(Medical Science)
基金
复旦大学遗传工程开放实验室部分资助
关键词
乳酸脱氢酶
基因表达
睾丸
杂交法
lactate dehydrogenase-C gene
. gene expression
ENA-DNA hybridization
