摘要
取54例经手术切除的肿瘤组织标本,用HRP-SPA法作神经元特异性烯醇酶(NSE)免疫组织化学染色。结果表明:在37例各种癌组织切片中,32例NSE染色阳性;在17例各种肉瘤组织切片中,3例NSE染色阳性。两者经x^2检验P<0.001,有极显著差异。NSE在正常情况下主要分布于神经元、神经内分泌细胞及APUD系统的细胞。在肿瘤情况下,除神经元、神经内分泌与APUD系统肿瘤的NSE染色为阳性外,非神经组织来源的肿瘤阳性率较高的有:肺、胃腺癌;肺、食道鳞癌;乳腺浸润性导管癌;肝细胞癌等。可供诊断与鉴别诊断的参考。提示:在肿瘤组织中NSE已失去其在正常情况下的分布特异性。
A commercially available monoclonal neuron specific enolase (NSE) antisernm (Polyscience)was tested against 37 cases of various types carcinomas and 17 different types of sarcomas using the HRP-SPA method. All the tumour tissue had baen fixed in formalin and embedded in paraffln. Positive reactions in tumour cells were found in 32 of the 37 carcinomas and three of 17 sarcomas. Under normal conditions, NSE is histoohemically demonstrable in neurons, neuroendoorine cells and colls of the APUD system. Although this shows a lack of specificity in tumour tissues, it is still a usful tool in the pathological diagnosis and differential diagnosis of malignant tumours.
出处
《上海医科大学学报》
CSCD
1990年第2期99-102,共4页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金1987准字第(3860953)
关键词
烯醇酶
肿瘤诊断
免疫组化
NSE
neuron specific enolase
imnmnohistochemistry
cancer
sarcomas
diagnosis
differential diagnosis
