摘要
目的：探讨将外周血单个核细胞（ＰＢＭＮＣ）的促血浆凝固活性（ＰＣＡ）用于急性排斥反应（ＡＧＲ）监测的可能性。方法：将新西兰白兔随机分为３组：第１组做自体皮肤移植（８只），第２、３组做异体皮肤移植（８、３只），第３组于术前１ｄ至术后７ｄ皮下注射环孢霉素Ａ５ｍｇ／（ｋｇ·ｄ）。术后每天观察移植皮片的变化。术前１ｄ及术后隔日取耳缘静脉血测ＰＣＡ和激活淋巴细胞计数，同时取移植皮肤组织作病理检查。结果：同种异体皮肤移植动物在出现ＡＧＲ前，其ＰＢＭＮＣ离体后不经培养即表现较强的促凝活性（ＳＰＣＡ），经供体抗原刺激后的ＰＣＡ（ＤＡｇＰＣＡ）活性也于ＡＧＲ前开始增强，而且表明具有抗原特异性。结论：ＰＣＡ检测方法在ＡＧＲ早期监测方面有一定的应用前景。
Objective: The sequential changes of procoagulant activity(PCA)
level in mononuclear cells (MNC) of rabbits which received skin allograft were studied in the
paper in order to assess it as a marker for monitoring of acute graft rejection (AGR). Methods:
New Zealand white rabbits(NZW)were random divided into three groups: rabbits in group
1(control) received autogenous skin transplants; rabbits in group 2, 3 received allogenic skin
transplants(a grey rabbit as a donor), and rabbits in group 3 were treated with cyclosporin A
(CsA) at 5 mg/(kgd) once daily subcutaneously for up to 9 days since the day before operation.
Heparinized peripheral blood was drawn from each animal on the day of the operation and
every other day after the operation. MNC were separated by FicollHypaque before PCA
measurement. The activated lymphocytes were counted at the same time. Morphologic changes
of the skin grafts were observed every day after transplant operation. Results: PBMNC of the
animals which received allograft had larger amounts of PCA before the day of AGR, in addition,
donor antigen during culture of PBMNC further enhanced PCA(DAgPCA) level in animals with
AGR. Conclusion: PCA may be as markers of AGR. DAgPCA is a specific marker.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第6期265-267,共3页
Chinese Journal of Immunology
基金
北京市科学技术委员会资助
