摘要
合成了生物素化壳聚糖(Bio-CS),并通过核磁共振(1HNMR)及电感耦合等离子体光质谱法(ICP-MS)对其进行结构确证.采用溶剂挥发法(W1/O/W2)制备聚乳酸-羟基乙醇酸(PLGA)纳米粒,并通过共价交联法对纳米粒进行Bio-CS表面修饰.未修饰的PLGA纳米粒在扫描电镜下观察呈规则球状形态,平均粒径为(248.4±21.0)nm,Zeta电势为-(21.21±2.13)mV,Bio-CS修饰后的PLGA纳米粒保持球状形态,平均粒径为(268.3±23.4)nm,Zeta电势为(25.45±2.59)mV.采用X射线光电子能谱(XPS)以及试剂盒对纳米粒表面生物素进行定性及定量研究,结果显示,经过Bio-CS修饰后的纳米粒表面含有N,S元素,生物素取代度为31%的Bio-CS修饰的PLGA纳米粒,其表面生物素含量为(1.36±0.34)μmol/100mg纳米粒.
Biotinylated chitosan（Bio-CS conjugates or Bio-CS） were synthesized and characterized.The degree of substitution（DS）,as defined as the number of biotin per 100 anhydroglucose units of CS,was determined by 1H NMR and ICP.Poly（lactic-co-glycolic acid）（PLGA） nanospheres were prepared by a solvent evaporation technique（W1/O/W2）.The surface of PLGA nanosphere was modified with Bio-CS by covalent binding.PLGA nanospheres were almost spherical in shape under the scanning electron microscopy（SEM） observation,and their mean diameter determined by the laser light scattering technique was （248.4±21.0） nm.Bio-CS modified PLGA nanospheres were also spherical in shape;their mean diameter and Zeta potential value were （268.3±23.4） nm and-（21.21±2.13） mV,respectively.The surface chemistry of nanospheres was quantitatively studied by X-ray photoelectron spectroscopy（XPS）.The results show the N region corresponding to the primary amide of CS and the S region corresponding to the biotin,indicating that PLGA nanospheres were successfully surface-modified with Bio-CS.The content of biotin on the surface of Bio-CS modified PLGA nanospheres with DS of biotin of 31% was determined using a commercially available QuantTagTM Biotin Kit and its value was （1.36±0.34） mol/100 mg.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第8期1682-1687,共6页
Chemical Journal of Chinese Universities
基金
国家重大科学研究计划项目(批准号:2006CB933300)
新乡医学院省级重点学科开放课题资助
关键词
纳米粒
聚乳酸-羟基乙醇酸
生物素化壳聚糖
抗肿瘤
Nanoparticle
Poly（lactic-co-glycolic acid）（PLGA）
Biotinylated chitosan anticancer
