摘要
目的:通过实验研究观察肾衰泻浊丸对腺嘌呤致肾间质纤维化大鼠的TGF-β1/Smads信号转导通路的影响,探讨肾衰泻浊丸抗肾间质纤维化的机理。方法:采用腺嘌呤致肾间质纤维化大鼠模型,观察治疗后实验大鼠肾间质纤维化情况;采用免疫组织化学法分别检测肾组织中的TGF-β1、Smad2/3蛋白的表达,采用Western Blot法检测肾组织内的Smad2/3蛋白的表达,采用半定量RT-PCR检测肾组织内的TGF-β1mRNA的表达水平。结果:肾衰泻浊丸能够减少肾间质纤维化面积;能够降低肾间质纤维化大鼠肾组织中TGF-β1,Smad2/3蛋白的表达,与对照组比较有显著性差异(P<0.05);能够下调肾组织中TGF-β1mRNA的表达,与对照组比较有显著性差异(P<0.05)。结论:肾衰泻浊丸可能是通过降低Smad2/3、TGF-β1的蛋白表达,从而抑制了TGF-β1信号向细胞核内转导的通路,这可能是其延缓肾间质纤维化进展的机制之一。
Objective： To investigate the effect of the Shenshuaixiezhuo Pill to TGF-β1 /Smads signal transduction pathway of adenine caused renal interstitial fribrosis rats,and to illustrate the mechanism of Shenshuaixiezhuo Pill on relieving renal interstitial fibroblast.Methods： In the experiments,the adenine caused renal interstitial fribrosis model is adopted.Masson staining method for pathologic slice are used to observe the pathlologic change of the experimental rats kidney tissue afer therapied.The protein expression of TGF-β1,Smad2 /3 in kidney tissue are detected respectively by immunohisto-chemistry.The proteins expression of Smad2 /3 are also detected by Western Blot method.The expression of TGF-β1mRNA is also detected by semi-quantitative RT-PCR.Results： Shenshuaixiezhuo Pill could decrease obviously the area of renal interstitial fribrosis.The Shenshuaixiezhuo Pill can adjusts the TGF-β1 and Smad2 /3 protein to low expression in kidney tissue,which has the significant difference compared with antithesis groups.The expression of TGF-β1mRNA are also reduced,which have significant difference with antithesis groups.Conclusions： The Shenshuaix-iezhuo Pill can possibly block TGF-β1 signal transduction by reducing TGF-β1 and Smad2 /3 protein expressions in kidney tissue.It is maybe one of mechanisms of Shenshuaixiezhuo Pill relieving renal interstitial fibroblast.
出处
《中医药学报》
CAS
2010年第4期22-25,共4页
Acta Chinese Medicine and Pharmacology
基金
哈尔滨市科技创新研究专项资金项目(编号:2006RFXYS043)
关键词
肾衰泻浊丸
肾间质纤维化
信号转导
TGF-Β1
SMAD蛋白
Shenshuaixiezhuo Pill
Renal interstitial fibroblast
Signal transfer
Transforming growth factor-β1
Smads
