摘要
目的探讨复元醒脑汤对高血压性脑出血大鼠脑水肿及血－脑屏障通透性的干预作用。方法将SHR大鼠随机分为四组，分别为假手术组、模型组、安宫牛黄胶囊组、复元醒脑汤组，并与WKY大鼠假手术组对照。每组20只大鼠。模型组、安宫牛黄胶囊组和复元醒脑汤组在收缩压达到21．3KPa基础上以胶原酶加肝素尾状核注射法诱发脑出血，建立高血压性脑出血模型，其中安宫牛黄胶囊组和复元醒脑汤组分别给予相应药物干预，于造模后连续灌胃3天。两假手术组脑内注射及灌胃均为生理盐水，模型组灌胃为生理盐水。观测各组动物神经缺损行为体征、脑组织含水量、血．脑屏障通透性变化。结果SHR模型组与WKY假手术组、SHR假手术组比较，神经缺损行为体征评分升高（均P〈0．01）；脑组织含水量增加（均P〈0．01），脑组织中伊文思蓝含量亦增加（均P〈0．01）；SHR复元醒脑汤和SHR安宫牛黄胶囊两治疗组分别与SHR模型组比较，神经缺损行为体征评分降低（均P〈0．01），脑组织含水量减少（均P〈0．01），脑组织中伊文思蓝含量亦降低（均P〈0．01）；上述指标变化复元醒脑汤组与安宫牛黄胶囊组比较差异无统计学意义（均P〉0．05）。结论复元醒脑汤可降低血-脑屏障通透性，减轻脑水肿程度，改善神经功能缺损．达到治疗高血压性脑出血的目的。
Objective To study the effect of Fuyuan Xingnao Decoction on Blood-brain Barrier Permeability in Hypertensive Cerebral Hemorrhage Rats.Methods Spontaneously Hypertensive Rats were randomly divided into four groups,sham operation grnup（SHRS,N=20）, model group（SHRM,N=20）,Angong Niuhuang capsule group（SHRTa,N=20）,and Fuyuan Xingnao Decoction group（SHRTf, N=20）,compared with Wistar Kyoto rats sham operation group（WKYS,N=20）.After the systolic pressure stabilizing in 21.3KPa,SHRM,SHRTa and SHRTf were induced cerebral hemorrhage by caudate nucleus injection with collagenase and heparin.Tben SHRTa and SHRTf were given corresponding drugs in intragastric administration for three days after getting into cerebral hemorrhage,while SHRS and WKYS were all given intraeerebral injection and intragastric administration with physiological saline,and SHRM was given intragastric administration with physiological saline.Rats of all groups were observed respectively their behavior and physical signs of neurologic defect,brain tissue moisture capacity,blood-brain barrier permeability.Results Compared with WKYS group and SHRS group,the SHRM group behavior and physical signs of neurologic defect grades,brain tissue moisture capacity and evans blue（EB） contents all increased up （P〈 0.01 ） ： SHRTa group and SHRTf group compared to SHRM group,behavior and physical signs of neurologic defect grades,brain tissue moisture capacity and EB contents all lowered （P 〈0.01 ） ; there is no statistical significant difference between SHRTa group and SHRTf group on the index changes （P〉 0.05） .Conclusion Fuyuan Xingnao Decoction may achieve the purpose of treating hypertensive cerebral hemorrhage through lowing blood brain barrier permeability,relieve cerebral edema, and improve neurologic impairment.
出处
《中国中医药现代远程教育》
2010年第17期206-208,共3页
Chinese Medicine Modern Distance Education of China
关键词
脑出血
脑水肿
自发性高血压大鼠
血-脑屏障
Cerebral hemorrhage
Cerebral edema： Spontaneously hypertensive rats
Blood-brain barrier
