摘要
经差速和蔗糖密度梯度离心，从Ｐ１０５９感染鸡的血液中提取巴氏杆菌。每羽鸡可提取较纯的菌液约１０ｍｌ，含菌量在１００亿／ｍｌ以上。该菌液灭活后免疫鸡，用异型菌Ｃ４８１交叉攻毒，结果与肝组织苗免疫的一样，均具有５０％的保护率，而Ｐ１０５９攻毒耐过鸡则全保护。结果证明，体内繁殖的巴氏杆菌具有交叉保护性，而体外培养菌则没有。
PasteurellamultocidaharvestedfromthebloodofexperimentallyinfectedchickenswithstrainP1059werepurifiedbydifferentialandsucrosedensitygradientcentrifugation.Thebacteriasuspensiongainedfromeachchickenwasabout10ml,thattheCFUisover101010.ThetrialchickensvaccinatedwiththiskilledbacteriasuspensionwerechallengedbyheterogeneousstainC481.Itshowedthattherateofcrossprotectionwas50%,andvaccinatedwithliverhomogenatespreparedfromthechickensdiedofinfectionbyP1059alsoThechickenssurvivedinfcetionbyliveP1059 werecrossprotectedcompletely.TheresultsdemonstratedthatPasteurellamultocidapropagatedinvivocoouldinducecrossprotection,andinvitronot.
出处
《中国预防兽医学报》
CAS
CSCD
1999年第3期185-186,共2页
Chinese Journal of Preventive Veterinary Medicine
基金
浙江省自然科学基金
