摘要
凤尾蕨Pteriscretica和Pteirsumbrosa孢子无菌接种于1／2MS大量元素和KP大量元素琼脂固体培养基上。置于温度20～22℃、光照强度为2200LUX连续光照的培养室中培养。用光镜及扫描电镜观察记载配子体及孢子体的发育过程及形态，以此确定配子体发育类型和配子囊个体发育特性。两凤尾蕨孢子培养5～7d后萌发，原叶体早期发育大致相近，与Oeratopteris发育类型类似：幼原叶体匙形具侧生分生区，同时，存在一些不规则的配子体发育类型，凤尾蕨Pteriscretica的一些个体的发育类型介于Adiantum和Ceratopteris类型之间，而P．umbrosa的一些个体介于Kaulina和Ceratopteris之间。培养25～33d后原叶体达到性成熟，P．cretica以雌性配子体为主，而P．umbrosa则以雄性配子体为主。配子囊发育属薄囊孢子型。孢子体无配子生殖。
Spores of the ferns Pteris cretica and P. umbrosa were aseptically cultured on 1/2 MS macronutrients and full Knop macronutrients solution solidified with Difco- Bacto Agar (6g/1 ).The cultures were maintained in a growth chamber under continuous fluorescent illumination of2200 LUX, temperature was 20 - 22℃. Development and morphology of gametophytes and sporophytes have been studied under light microscopc and SEM to determine the gametophytic development type and the ontogenetic sequence of gametangia expression. Sproes germinated 5 - 7 days after sowing. The prothallia development is of the Ceratopteris type by the development of a spatulate with lateral meristemic sone. There were also some individuals were intermediate type and abnormal prothallus: in P. cretica most individuals were intermediate between the Adiantum and the Ceratopteris. In P. umbrosa some individuals are intermediate between the Kaulina and Ceratopteris. The Prothallus reached sexual maturity in 25 - 33 days. In p. cretica. gametophytes were predominantly females, and in P. umbrosa, were abundant in male gametophytes.The gametangia are of the common leptosporangiatetypc. Sporophytes regenerated apogamously.
出处
《湖南林业科技》
北大核心
1999年第1期1-7,共7页
Hunan Forestry Science & Technology
基金
湖南省中青年科技基金
关键词
凤尾蕨
配子体
孢子体
繁殖
生物学
观叶植物
Pteris cretica Pteris umbrosa, Gametophyte Soprophyte Apogamous propagation
