摘要
目的研究淫羊藿苷(ICA)单体对猪体外受精早期胚胎体外发育过程中培养液内一氧化氮(NO)和丙二醛(MDA)生成量的影响,探讨其与猪胚胎体外发育效果的关系。方法以NCSU-23培养液为对照组,以添加0.6mg/L淫羊藿苷为实验组(ICA组),通过硝酸还原酶法和硫代巴比妥酸法测定猪体外受精卵体外培养0～48h、48～120h、120～168h培养液滴中NO和MDA水平。结果 ICA组(342枚受精卵)48h卵裂率(74.84%vs63.32%)、120h桑囊率(45.08%vs34.10%)、168h囊胚率(23.38%vs18.04%)均极显著高于对照组(349枚受精卵)(P<0.01)。在猪体外受精卵体外培养0～168h整个过程中,培养液内NO、MDA水平总体均呈上升趋势,但在胚胎发育3个阶段中ICA组平均每枚胚胎NO生成量高于对照组,MDA生成量则低于对照组(P>0.05)。结论 ICA有利于促进猪体外受精早期胚胎的体外发育,可能与其在一定程度上提高NO水平,并同时抑制MDA的生成有关。
Objective To study the effects of icariin （ICA） on the level of nitric oxide （NO） and malondialdehyde （MDA） in culture medium of fertilization（IVF） early porcine embryos in vitro, and to research both the relations among ICA, NO, MDA and the association with developmental ability of porcine IVF embryos. Methods NCSU-23 basic culture medium was used as the medium for control group, while ICA（0. 6mg/L）-supplemented medium was used as the medium for the experiment group. The reductive ferment method and thiobarbituric acid colormetry method were used to detect the nitric acid and MDA in media at interval duration of culture （0-48h,48-120h, 120-168h）. Results There were significantly different between experimental （342 embryos） and control （349 embryos） groups in the cleavage rate （74. 84 vs 63.32% ） （ICA vs control） , the morrula and blastoeyst rate （45.08 vs 34.10 % ） , and the blastocyst rate（23.38 vs 18.04% ） at the intervals for 48 th, 120 th, 168 th hour（P 〈 0.01 ）. During the culture process of porcine embryos, the concentrations for both NO and MDA in media kept increasing, while NO concentration in medium was higher in ICA group than that in the control group, the MDA concentration in medium was lower in ICA group than that in the control group, but there was no statistic significant（P 〉 0. 05）. Conclusion ICA can promote the developmental ability of porcine IVF early embryos, which may be relevant to the up regulation of NO level and down regulation of MDA level.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第4期623-625,共3页
Acta Anatomica Sinica
基金
北京市教委资助项目(KM200710020014)
北京市教委中药添加剂创新团队项目
关键词
淫羊藿苷
一氧化氮
丙二醛
胚胎
体外发育
体外受精
猪
Icariin
Nitric oxide
Malondialdehyde
Embryo
In vitro development
In vitro fertilization
Pig
