摘要
目的:通过对十堰市外环境水体霍乱弧菌的检测分析,了解霍乱弧菌在外环境中的生存变化与流行的关系、掌握霍乱的流行规律、预测流行趋势,为制定防治措施提供依据。方法:采集外环境监测点水样,按国标法进行霍乱弧菌分离,PCR检测毒力基因,用ATB进行生化鉴定和耐药性分析。结果:从生活污水中检出1株O139群霍乱弧菌非流行株,该菌株对阿莫西林等17种抗菌素敏感;对环丙沙星中度敏感;对哌拉西林、复方新诺明耐药。结论:十堰市自然水体中存在有O139群霍乱弧菌,提示在今后的霍乱监测中,这些环境应作为监测重点,0139群霍乱弧菌多重耐药应引起重视。
Objective：To know the relationship of exist,vary and prevalence of Vibrio cholera in environment,master the law of cholera epidemics,predict trends in cholera epidemics,provide basis for drawing down prevention and control strategy,by detecting and analysising of Vibrio cholera in environmental water in shiyan city.Methods：Environmental water was collected,isolated by the procedure from national standard,then virulent genes were amplified by PCR,biochemical identification and drug-resistant analysis carried out with ATB.Results：One strain of Vibrio cholera O139 was isolated from sewage,which was sensitive to 17 kinds of antibiotics include ampicilin,moderately susceptible to ciprofloxacin,tolerant to piperacillin and cotrimoxazole.Conclusion：Vibrio cholera exist in natural water in shiyan city,this kind of environment should be focused on in future surveillence of Cholera,multi-drug-resistant of Vibrio cholera should be paid more attention to.
出处
《中国卫生检验杂志》
CAS
2010年第7期1615-1616,1620,共3页
Chinese Journal of Health Laboratory Technology
关键词
外环境水
霍乱弧菌
鉴定
耐药分析
Environment water
Vibrio cholera
Identification
Drug resistent analysis
