摘要
通过正交试验,考察了酸-菌-酶法共降解黄姜皂素生产废渣的最佳糖化工艺条件。结果表明,皂素生产废渣先进行酸水解,再利用黄孢原毛平革菌对其中的木质素进行降解,最后进行酒精酶水解,得到这种酸-菌-酶法糖化工艺的最优条件为,蒸汽处理后的废渣先经液固比20∶1、稀硫酸(w=4%)、100℃的条件下酸水解120 min;再于30℃、pH6、加菌量100 mL.L-1的培养条件下利用黄孢原毛平革菌(Phanerochaete chry-sosporium)降解4 d;最后在酶用量0.9 mL、温度为50℃、pH4.8的工艺条件下酶解100 min。黄姜皂素废渣经该条件处理后,木质素去除率达42.82%,还原糖得率最高达59.72%,比采用酸-酶法水解后的还原糖得率提高了25.62%。这种酸-菌-酶法结合工艺可以更有效地降解皂素生产废渣,得到较高的还原糖量。
The orthogonal test was applied to examine the optimal saccharification process conditions by the method of acid-microorganism-enzyme co-degradation for the residue of Dioscorea zingiberensis saponin production.The results showed that the optimal conditions of the acid-microorganism-enzyme saccharification technology were as follows.At first,saponin production residue after the high-temperature steam treatment was hydrolyzed by acid under the best conditions of 100℃,ratio of liquid-solid 20：1,the dilute sulfuric acid concentration 4%,hydrolysis time for 120 min;then degraded with Phanerochaete chrysosporium for four days under the optimum conditions of 30℃,pH 6,and the amount of microorganism 100 mL·L-1.After fermentation,waste residue was continued to further hydrolyze by alcohol enzyme under the conditions of quantity of enzyme 0.9 mL,temperature 50℃,pH 4.8,and enzymatic hydrolysis time 100 min.After the saponin production residue was treated under the best conditions,lignin removal rate reached 42.82%,and reducing sugar yield was up to 59.72% which was 25.62% higher compared with the treatment of acid-enzyme hydrolysis.This acid-microorganism-enzyme combined process can be more effective in degradation of saponin production residue and the higher amount of reducing sugar can be obtained.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第7期196-201,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家环保示范项目(财建[2004]278号)
关键词
皂素生产废渣
酸-菌-酶法
糖化
还原糖
Saponin production residue
Acid-microorganism-enzyme co-degradation
Saccharification
Reducing sugar
