摘要
对陕西省杨凌区某奶牛场经体细胞计数(SCC)法确定为隐性乳房炎的20份乳样中的主要病原菌进行分离鉴定,并对分离菌进行耐药性测定。共分离到24株分离菌,其中金黄色葡萄球菌10株,占41.6%;沙门氏菌6株,占25.0%;表皮葡萄球菌5株,占20.8%;大肠埃希菌2株,占8.4%;其他细菌1株,占4.2%。耐药性试验显示,主要分离菌均对庆大霉素和卡那霉素敏感,而对四环素、复方新诺明有较强的耐药性,链霉素、红霉素、氯霉素对分离菌的生长也有明显的抑制作用,建议在临床治疗中联合用药,以取得更好的效果。
The main bacterial pathogen were isolated from 20 cow milk samples,which were collected from 20 subclinical mastitis cows demonstrated with somatic cell count（SCC） test,resistance detection was performed in isolated bacteria.The results showed that 24 bacteria were isolated from clinical milk samples,including ten Staphylococcus aureus,six Salmonella,five Staphylococcus epidermidis,two Escherichia coli and one other bacterium,the detection rate of bacteria were 41.6%,25.1%,20.8%,8.4% and 4.2%,respectively.The experiments of resistance to medicine demonstrated the main isolates of bacterium were susceptible to Gentamicin and Kanamycin,but they were resistant to Tetracycline and TMP/ SMZ.Streptomycin,Erythromycin,and Chloramphenicol had an inhibitory on the isolated bacteria.It is a better way to prevent and therapy mastitis in cow with drug combination.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第7期30-34,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省重大科技攻关项目(2006KZ07G2)
关键词
奶牛
隐性乳房炎
病原菌
分离鉴定
耐药性
Dairy cattle
Subclinical mastitis
Bacterial pathogen
Isolation and identification
Drug resistance
