摘要
采用毕赤酵母偏爱原则,人工设计合成抗菌肽Spinigerin基因。按正确的阅读框架定向克隆至真核表达载体pPICZαA,经PCR鉴定及序列分析,所转化的毕赤酵母GS115中含有Spinigerin插入基因的重组质粒pPICZαA-S,结果表明成功构建了抗菌肽Spinigerin基因真核表达载体pPICZαA-S。
The antibacterial peptide Spinigerin gene was designed and artificially synthesized by the biased codon usage of yeast. Antibacterial peptide Spinigerin gene was subcloned into shuttle expression vectors pPICZα A, by using PCR and sequence analysis, it can be proved that all the tested clones contain Spinigerin gene, pPICZαA -S is successfully constructed.
出处
《食品与发酵科技》
CAS
2010年第4期79-81,共3页
Food and Fermentation Science & Technology
基金
吉林省重大科技资助项目(20060208)
