摘要
取健康青壮年男性献血员的新鲜外周静脉血,肝素抗凝,经密度梯度离心获得单个核细胞。又经粘附法和尼龙毛柱分离法分别获得高纯度的单核细胞和T淋巴细胞。用单克隆抗体分别阻断单核细胞表面的 HLA-DR、HLA-DQ、HLA-DP 抗原,经洗涤后,将单核细胞与PPD共同温育24小时,观察单核细胞向T淋巴细胞提呈PPD的能力。另一组先将单核细胞与 PPD作用24小时,然后用单克隆抗体阻断单核细胞表面的 IL-2R1小时,再观察单核细胞的抗原提呈功能。结果表明,分别阻断单核细胞表面的 HLA-DR、DQ、DP 和IL-2R 后,其抗原提呈能力均显著减弱。
Mononuclear cells separated from heparinized fresh peripheral venous blood of healthy male blood donors by means of Ficoll-Hypaque density gradient centifugation were subjected to Petri dish adhesion and nylon wool-column separation respectively to obtain monocytes and T, lymphocytes of high purity. The capability of the monocytes to present the antigen PPD(purif ied protein derivative. Behring Institute, West Germany, end concentration 12. 5μg/ml) to T lymphocytes, was assayed by 3H-TdR incorporated T lymphocyte proliferative reaction. It was shown that blocking of the HLA-DR, HLA-DQ HLA-DP antigens and interleukin-2 receptors on the monocytes separately with cori es ponding monoclonal antibodies (The Wuhan Institute of Biologic Products) resulted in marked depression of the antigen-presenting capability of the monocytes, Suggesting that all three kinds of molecules of the human Ia antigen, as well as the inter-leukin-2 receptors on the monocyte surface are involved, in the function of antigen presentation.
出处
《上海免疫学杂志》
CSCD
北大核心
1990年第3期129-132,共4页
Shanghai Journal of Immunology
基金
国家自然科学基金
