摘要
本研究检测儿童急性白血病患儿急性髓系白血病干细胞(AMLLSC)的水平,分析AML患儿缓解后白血病干细胞(leukemia stem cells,LSC)含量与微小残留病(MRD)水平之间的关联。采用流式细胞术(FCM)和直接免疫荧光法标记细胞,分别测定初诊AML、ALL和缓解AML、ALL及对照组患儿骨髓单个核细胞(mononuclear cell,MNC)中AMLLSC(CD34+CD38-CD123+)表型阳性细胞的含量及AML患儿缓解后MRD水平。结果表明:初诊AML、ALL及对照组患儿骨髓MNC中AMLLSC或AMLLSC相同表型细胞的中位含量分别为166(14-1459)细胞/10万细胞、7(0-560)细胞/10万细胞和0(0-6)细胞/10万细胞,未缓解AML患儿骨髓MNC中AMLLSC的中位含量为36(5-224)细胞/10万细胞,缓解AML、ALL患儿骨髓MNC中AMLLSC或AMLLSC相同表型细胞的中位含量分别为6(0-41)细胞/10万细胞和10(0-105)细胞/10万细胞,缓解期AML患儿AMLLSC含量与同期MRD水平呈负相关(r=-0.466,p=0.006)。结论:初诊AML患儿骨髓细胞中存在AMLLSC,缓解后AMLLSC含量明显减少,维持一个较低水平;在初诊ALL患儿骨髓细胞中也存在AMLLSC(CD34+CD38-CD123+)相同表型细胞,缓解后AMLLSC相同表型细胞含量没有明显变化;AML患儿缓解后AMLLSC含量与MRD水平呈负相关。
The aim of this study was to detect the presence of human AML leukemia stem cells （LSC） in childhood patients with acute leukemia （AL） and analyze the correlation between LSC concentrations and minimal residual disease （MRD） levels in AML cases after remission. The multi-parameter flow cytometry （FCM） and a panel of monoclonal antibody combination were used to detect the AML LSC or AML LSC immunophenotype-identical cell （AML LSC- IPIC） concentrations in childhood AML or ALL leukemia both at new diagnosis and at remission and correlated AML LSC to the MRD levels at different time points after remission. The results indicated that the AML LSC or AML LSC- IPIC concentrations [ in average 166 （range 14 - 1459 ）/100 000 mononuclear cells （MNCs）] in AML at initial diagnosis were significantly higher than those in ALL [ 7 （range 0 -560）/100 000 MNCs, p 〈 0.017 ] and control [ 0 （range 0 -6）/100 000 MNCs, p 〈 0. 017], respectively. The AML LSC concentrations in AML at non-CR were in average 36 （range 5 -224）/100 000 MNCs. No statistical difference （p 〉0.05） was found between the AML LSC or AML LSC-IPIC concentrations in AML （ in average 6 （ range 0 - 41 ）/100,000 MNCs） and ALL [ 10 （ range 0 - 105 ）/ 100,000 MNCs ] after CR. The significantly negative correlation was noticed between AML LSC concentrations and MRD levels. It is concluded that the AML LSCs exist in newly diagnosed AML, which are significantly reduced when complete remission has achieved, but the low levels of these populations still remain. The phenotypicaUy similar （CD34^＋ CD38^- CD123^＋ ） AML LSC populations have also been found in the bone marrow from ALL patients, but their concentrations are not significantly different when CR has achieved. The significantly negative correlation between AML LSC concentrations and MRD levels is observed in AML patients after remission.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第4期952-958,共7页
Journal of Experimental Hematology
基金
浙江省科技厅科技项目
编号:2007C23007
