摘要
目的探讨抑癌基因PTEN在口腔鳞状细胞癌发生、发展过程中的作用。方法应用聚合酶链反应-单链构象多态性分析(PCR-SSCP)方法 ,扩增76例口腔鳞癌PTEN基因的第5～8外显子,根据构象改变检测其基因突变情况。结果 PTEN基因的第5、第6及第7、8外显子均未发现有突变及缺失。结论在DNA水平抑癌基因PTEN在口腔鳞状细胞癌的发生过程中不起作用。
Objective To investigate the role of the tumor suppressor PTEN in the tumorigenesis and development of oral squamous cell carcinoma（OSCC）.Methods Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to amplify 4exons of PTEN and to analyze the conformation polymorphism,then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in OSCC.Results There was no mutation and deletion in exon5,exon6,exon7 and exon8 of PTEN gene in OSCC and normal mucous membrane.Conclusion At the level of DNA,PTEN do not play any roles in OSCC.
出处
《实用心脑肺血管病杂志》
2010年第9期1241-1242,共2页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
关键词
口腔鳞癌
PTEN基因
突变
Oral squamous cell carcinoma
Anti-oncogene PTEN
Mutation
