摘要
以热研88-13×IAN873的94个F1群体为试材,利用简单序列重复(Simple sequence repeat,SSR)标记,采用FsLinkage MAP 1.0软件,构建了巴西橡胶树热研88-13×IAN873的遗传连锁图谱。从441对SSR引物中筛选出160对具有多态信息的引物,在分离群体中共检测到206个多态性位点,176个位点用于遗传图谱的构建;χ2检验结果显示,有147个位点符合1:1分离比例,有12个符合1:2:1分离比例,有17个符合1:1:1:1的分离比例,共有13个偏分离位点,偏分离率低(7.38%);91个SSR位点被分为18个连锁群,覆盖橡胶树基因组1 937.06 cM,每个连锁群包含2～16个位点,标记间的平均距离为21.29 cM。
Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers,176 were used for constructing the genetic map of rubber tree（Hevea brasiliensis） by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873.Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1：1 and 12 loci followed a ratio of 1：2：1 and 17 loci followed a ratio of 1：1：1：1.Only 13（7.38%） loci were distorted from the Mendelian ratio.The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers.The largest linkage group consisted of 16 marker loci,while the smallest one contained only 2 marker loci.
出处
《遗传》
CAS
CSCD
北大核心
2010年第8期857-863,共7页
Hereditas（Beijing)
基金
中央级公益性科研院所基本科研业务费项目(编号:ITBBZD0715)资助
关键词
巴西橡胶树
SSR标记
遗传图谱
rubber tree（Hevea brasiliensis）
SSR markers
genetic lingkage map
