摘要
将纳米纤维应用于水中环境雌激素的富集与测定。对一些潜在的影响因素如纳米纤维的直径、离子强度、水样pH值进行了优化.提出了纳米纤维预富集、HPLC检测的分析水中邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)、双酚A的方法。该方法的线性范围为20-1000 ng/mE,检出限为1.0-2.5ng/mL(S/N=3),相对标准偏差(RSD)为3.97%-7.87%,N=5,提取回收率(绝对回收率)为57.9%-83.3%。该方法适合水样中环境雌激素的分析,且样品预处理方法更加快速、灵敏、简单、价廉,符合无(少)溶剂的样品前处理发展趋势。
Nanofibers were novelly applied to the pretreatment of environmental estrogens in the water samples based on the adsorption ability due to their large surface areas.The method for extraction was validated,with the optimization of involved parameters such as nanofiber diameter,ionic strength of sample solution and pH value. Under optimized conditions,the good linearity of analytes was achieved in the range of 20～1000 ng/mL.The repeatability of extraction performance,which is expressed as relative standard deviations,was 3.97%～7.87% corresponding to the water samples at 50～500 ng/mL spiked level for six kinds of compounds.The limits of detection （LODs） varied from 1.0 ng/mL to 2.5 ng/mL （S/N=3）.The absolute recoveries were 57.9%--83.3%.The new method reduces the sample preparation time,the organic solvent consumption,and the damage to persons and environment,and improves the sensitivity and reproducibility for the investigated compounds.The present results indicate that the new method is a viable and environmentally friendly technique for analyzing environmental estrogens.
关键词
纳米纤维
环境雌激素
预处理
环境水样
nanofibers
environmental estrogens
pretreatment
environmental water samples
