摘要
目的：探讨哮喘时嗜酸性粒细胞（Ｅｏｓ）浸润与ＩＬ－５ｍＲＮＡ表达的关系以及糖皮质激素对其影响。方法：采用孵蛋白（ＯＡ）吸入致敏制作豚鼠哮喘模型，在诱发哮喘２４ｈ后，检测支气管肺泡灌洗液（ＢＡＬＦ）中不同密度的Ｅｏｓ的数量及其百分比，肺组织中原位ＩＬ－５ｍＲＮＡ的表达，以及地塞米松预防用药６ｄ对其变化的影响。结果：①哮喘组ＢＡＬＦ中Ｅｏｓ总数增加，低密度Ｅｏｓ（ＨＥｏ）增加尤为显著；肺组织中ＩＬ－５ｍＲＮＡ原位表达水平显著增加（Ｐ＜０．０１）；②与哮喘组比，地塞米松预防用药后Ｅｏｓ计数及ＨＥｏ百分比显著减少（Ｐ＜０．０１）；③相关分析发现，ＩＬ－５ｍＲＮＡ表达水平与Ｅｏｓ总数（ｒ＝０．６８８，Ｐ＜０．０１）及ＨＥｏ百分比显著正相关（ｒ＝０．５８２，，Ｐ＜０．０１）。结论：哮喘豚鼠肺组织ＩＬ－５ｍＲＮＡ表达的增加与ＢＡＬＦ中Ｅｏｓ数量增多密切相关。抑制ＩＬ－５ｍＲＮＡ表达可能是糖皮质激素减少Ｅｏｓ浸润及治疗支气管哮喘的重要机制之一。
Objective: To explore the relationship between interleukin 5 (IL 5) mRNA expression and eosinophil (Eos) infiltration in the lung tissue of asthmatic guinea pigs and the effects of glucocorticoid on it. Methods: Guinea pigs were randomized into the control group (CG), asthmatic group (AG) and dexamethasone pretreated group (DPG). The animals in AG were sensitized by exposure to aerosolized ovalbumin to establish the model of asthma and those in DPG were treated likewise except for intraperitoneal injection of dexamethasone 6 h before the exposure. Twenty four hours later, the counts of total Eos, hypodense eisinophil (HEo) and normal dense eosinophil (NEo) in the bronchoalveolar lavage (BALF) were measured. The changes in the percentage of HEo and NEo, IL 5 mRNA in situ expression and the effects of dexamethasone on them were determined. Results: ① The counts of total Eos and the percentage of HEo in BALF and IL 5 mRNA expression in the lung tissue in AG were significantly increased ( P <0.01) as compared with CG. ② The three parameters in DPG were significantly reduced in DPG as compared with AG ( P <0.01). ③ IL 5 mRNA expression was significantly correlated with the counts of total Eos ( r =0.688, P< 0.01) and percentage of HEo ( r= 0.582, P <0.01) in BALF. Conclusion: The enhancement of IL 5 mRNA in the lung tissue is closely associated with the increase in total counts of Eos. Inhibition of IL 5 mRNA expression in the lung tissue might be one of the important mechanisms of using glucocorticoid to reduce infiltration of Eos in the lung tissue to treat asthma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第2期93-96,共4页
Journal of Third Military Medical University
关键词
哮喘
哮酸性粒细胞
IL-5
MRNA
地塞米松
asthma
eosinophil
interleukin 5
in situ hybridization
dexamethasone
guinea pig
