摘要
目的探讨三氧化二砷（Ａｓ２Ｏ３）对急性早幼粒细胞性白血病（ＡＰＬ）细胞组织因子（ＴＦ）表达的影响。方法利用复钙时间检测使用１μＭＡｓ２Ｏ３处理ＡＰＬ细胞株ＮＢ４细胞以及使用Ａｓ２Ｏ３治疗中不同时间的ＡＰＬ病人的骨髓细胞的促凝活性（ＰＣＡ），并用ＥＬＩＳＡ方法检测其ＴＦ抗原、利用半定量ＲＴＰＣＲ检测ＴＦｍＲＮＡ的转录水平。结果Ａｓ２Ｏ３可以以时间依赖的方式下调ＡＰＬ细胞的促凝活性、ＴＦ抗原水平以及ＴＦｍＲＮＡ的转录；此外，通过对ＰＣＲ产物进行测序，发现ＡＰＬ细胞中还存在ＴＦ第五个外显子缺失的转录本，其生物学意义尚不明了。结论Ａｓ２Ｏ３可下调ＡＰＬ细胞ＴＦ的表达；而且在使用Ａｓ２Ｏ３诱导ＡＰＬ细胞凋亡的同时。
Objective To investigate the effects of arsenic trioxide (As 2O 3) on tissue factors(TF) expression of acute promyelocytic leukemia(APL) cells. Methods Procoagulant activity(PCA) of APL cell line NB4 cell treated with 1μM As 2O 3 and freshly isolated bone marrow blasts from APL patients treated with As 2O 3 were detected using a one stage clotting assay, as well as TF antigen detected by ELISA, TF mRNA by semi quantitative RT PCR. Results As 2O 3 can time dependently downregulate the TF antigent, its mRNA transcription and membrane PCA of APL cells. Moreover, by dideoxy sequencing of DNA fragment derived from PCR, it was found that there was an exon 5 deletion transcript of TF in APL cell as well, its biological significance remained unknown. Conclusion Tissue factors expression of APL cells may be downregulated by As 2O 3, and by downregulating the TF expression, As 2O 3 may also be used as a preventive agent for DIC in APL during its induction of APL cells to apoptosis.
出处
《上海医学》
CAS
CSCD
北大核心
1999年第2期91-94,共4页
Shanghai Medical Journal
基金
胡应洲基金
