摘要
目的:建立一种能够快速、有效地对大鼠和家兔胎仔骨骼进行批量单染和双染的方法。方法:通过制作染色用具及建立染色程序,对大鼠和家兔胎仔进行单染和双染,并对新建方法与常规方法的染色效果进行比较。结果:新建的骨骼染色方法能够减少工作量,节约耗材,且染色效果与常规方法相同。结论:这种新方法可应用于药物的安全评价。
OBJECTIVE：To develop a new method for quick,effective and batch-wise skeletal staining of the fetuses of rats and rabbits.METHODS：After setting up of relevant staining equipments and improvement of staining procedures,the fetuses underwent single and double skeletal staining by the new batch-wise procedures,and the staining quality of fetal skeletons was compared with those stained with routine procedures used in our lab.RESULTS：With the new method for skeletal staining,the workload for the staff and consumables used could be reduced,with comparative staining effects as those for the routine procedures.CONCLUSION：This batch-wise skeletal staining procedure could be used in drug safety evaluation.
出处
《癌变·畸变·突变》
CAS
CSCD
2010年第4期295-297,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
北京市自然科学基金(7092079)
北京市新药研发系统创新服务平台建设(Z08030203080818)
重大新药创制科技重大专项(2008ZX09305-003)
关键词
胎仔骨骼
批量染色
单染法
双染法
大鼠
家兔
fetal skeleton
batch-wise staining
single staining
double staining
rat
rabbit
