摘要
为探讨淋巴细胞表面Ｆａｓ及其配体（ＦａｓＬ）在ＩＬ－２和ＩＬ－１２作用下表达的变化，以及Ｆａｓ和ＦａｓＬ与淋巴细胞抗肿瘤活性的关系，作者用免疫荧光标记和流式细胞仪分析了ＩＬ－２和ＩＬ－１２激活的杀伤细胞（ＩＬ－２ＡＫ和ＩＬ－１２ＡＫ）Ｆａｓ和ＦａｓＬ的表达；分别用乳酸脱氢酶（ＬＤＨ）释放法和ａｎｎｅｘｉｎ－Ｖ－ＦＩＴＣ标记检测ＩＬ－２ＡＫ和ＩＬ－１２ＡＫ杀伤的肿瘤细胞的坏死和凋亡。结果表明：①正常人外周血淋巴细胞（ＰＢＬ）（２０．４０±５．６９）％表达Ｆａｓ，但无ＦａｓＬ表达；３株消化道肿瘤细胞系恒定表达Ｆａｓ，始终不表达ＦａｓＬ；②ＩＬ－２与ＩＬ－１２可诱导ＬＡＫ细胞表达ＦａｓＬ（Ｐ＜０．０１），并促使Ｆａｓ表达显著增加（Ｐ＜０．０１）；③ＬＡＫ细胞致肿瘤细胞死亡中ＦａｓＬ表达与ａｎｎｅｘｉｎ－Ｖ标记阳性率和肿瘤细胞表面Ｆａｓ表达正相关。由此推论，Ｆａｓ－ＦａｓＬ途径是ＩＬ－２与ＩＬ－１２诱导活化的ＬＡＫ细胞杀伤肿瘤的途径之一，淋巴细胞致肿瘤凋亡分别与淋巴细胞ＦａｓＬ表达和肿瘤细胞Ｆａｓ表达一致。
To determine the expression of Fas and Fas ligand (FasLor CD95L) on lymphocytes which were activated by IL 2 and IL 12, and to bring light the relationship between their expression and the anti tumor activity of the lymphocytes. Fas and FasL were labeled with immunofluorescence, and determined by flow cytometer. LDH release and annexin V Fluo /PI labeling were used, respectively, to detect the necrosis and the apoptosis of tumor cells. The results showed that ①Lymphocytes from normal donors carried (20.4±5.69)% of Fas and nor FasL, three tumor cell lines expressed constantly Fas but no FasL;②IL 2 and IL 12 could induce the expression of FasL and significantly increased the Fas expression on LAK cells;③The FasL expression was positively correlated with the positive rate of annexin V labeling and Fas expression on tumor cells killed by the LAK cells. It was concluded that the expression of FasL on IL 2AK and IL 12AK and the Fas on tumor cells had positive correlation with the apoptosis of tumor cells; and Fas and FasL was one of pathways that IL 2AK and IL 12AK killed tumor targets.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1999年第1期28-30,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
淋巴细胞
FAS
FASL
抗肿瘤活性
白细胞介素
antigens, CD95
ligands
killer cells, lymphokine activated
tumor cells, cultured
apoptosis
