摘要
大量功能好的肝细胞是生物人工肝支持系统(bio-artificial liver support system,BLASS)的核心,是制约BLASS临床广泛应用的瓶颈.探索出一种实用的肝细胞低温保存方法,建立一个随时可用(ready to use)的肝细胞库是BALSS普遍推广的基础.目前肝细胞低温保存分为4℃或零下非结冰保存和-80℃或-196℃深低温冻存两大类,两大类保存方法各有优缺点.影响肝细胞低温保存的因素很多,如保存(冻存)液、(冻存)保护剂等.有关肝细胞在低温保存过程中死亡的机制尚未完全阐明,但大量研究发现细胞凋亡是除了坏死之外低温保存肝细胞死亡的另一个重要的途径.
The problem that high-quality hepatocytes are difficult to obtain restricts the use of bioartificial liver support system （BLASS） in clinical practice.Finding an effective way to preserve hepatocytes and constructing a ＂ready-to-use＂ hepatocyte bank would efficiently promote the development of the BLASS.Nowadays,the methods for hypothermic storage of hepatocytes could be classifiedinto two types：conventional hypothermic storage at 4°C or subzero nonfreezing storage,and cryopreservation at-80°C or-196°C.Each type of hypothermic storage method has its advantages and disadvantages.Many factors may affect the effect of hypothermic storage （cryopreservation）,such as storage solution and cryoprotective agent.Although the precise mechanism underlying the death of hepatocytes during hypothermic storage is not well understood,numerous studies have indicated that apoptosis plays an important role in hypothermic storage injury.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第17期1792-1798,共7页
World Chinese Journal of Digestology
基金
国家高技术研究发展计划(863计划)基金资助项目
No.2006AA02A141
全军医学科研"十一五"计划专项基金资助项目
No.08Z017~~
关键词
生物人工肝
低温保存
细胞凋亡
Bioartificial liver support system
Hypothermic storage
Cell apoptosis
