摘要
目的 分析慢性乙型肝炎（CHB）患者外周血及肝组织T淋巴细胞受体（TCR）β链各家族互补决定区3（CDR3）谱系的变化,了解CHB患者T淋巴细胞克隆增生的情况.方法 采用荧光定量PCR（FQ-PCR）溶解曲线法分析4例健康对照者及11例CHB患者外周血单个核细胞（PBMC）及肝组织T淋巴细胞受体β链可变区（TRBV）基因各家族CDR3谱系漂移情况,比较11例CHB患者TCR β链可变区各家族外周血及肝组织CDR3谱系漂移异常率.率的比较采用卡方检验.结果 11例CHB患者PBMC及肝组织TRBV各家族CDR3溶解曲线谱型图出现数量不等、形态不一的单峰、寡峰、偏峰及极低水平或缺失等现象,肝组织TRBV家族CDR3的谱系漂移异常率明显高于外周血的异常率（x2=23.246,P＜0.01）,在同一患者的外周血及肝组织中发现部分相同的TRBV亚家族表达,TRBV13.1、BV17和BV22同时被多例CHB患者的外周血及肝组织限制性取用.结论 CHB患者PBMC及肝组织中的T淋巴细胞均存在不同程度的克隆增生,部分TRBV亚家族被多例CHB患者外周血及肝组织同时限制性取用.
Objective To analyse the spectral patterns of complementarity determining region 3 （CDR3） length distribution of T lymphocyte receptor beta chain variable （TRBV） gene families in infiltrating T cells of the liver tissues and the peripheral blood samples of patients with chronic hepatitis B （CHB） in order to evaluate the characteristics of T cell clonal expansion. Methods The spectral patterns drift of TRBV gene families （the monoclonal/oligoclonal TCR β T cells） in the peripheral blood and hepatic tissues from 11 cases of CHB patients were analyzed by the real-time fluorescence quantitative reverse transcription polymerase chain reaction （FQ-PCR） with DNA melting curve analysis, and abnormal rates of TRBV gene families were compared between CHB patients and healthy control. The comparison of rates was done by chi square test. Results The gene melting spectral pattern of 26 TRBV families of the 11 CHB patients, no matter in the peripheral blood or hepatic tissue, showed either a single peak or prominent melting peaks, even disappeared for certain TRBV families. The abnormal rate of TRBV gene families in the hepatic tissues was significantly higher than that in the peripheral blood samples （x2 = 23. 246, P〈0. 01）. What is more interesting was that some parts of TRBV families were identical in both the peripheral blood and the hepatic tissue in certain patients. TCR BV13.1, TCR BV17 and TCR BV22 fragments were found to be restricted used in both the peripheral blood and hepatic tissue by some CHB patients. Conclusions T cells in the peripheral blood and the hepatic tissues of CHB patients can develop clonal expansion to some extent.Parts of TRBV families are restricted used in the peripheral blood and hepatic tissue in some CHB patients, which offers a foundation for further studying the common specific spectral drift patterns of TRBV CDR3 gene in CHB patients.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2010年第6期348-353,共6页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金项目（30760231＆30660172）
贵州省优秀科技教育人才省长专项基金项目[黔省专合字（2008）111号]
关键词
肝炎
乙型
慢性
受体
抗原
T细胞
α-β
互补决定区
聚合酶链反应
单核细胞
Hepatitis B, chronic
Receptors, antigen, T-cell, alpha-beta
Complementarity determining regions
Polymerase chain reaction
Monocytes
