成年期甲减对大鼠额叶SNAP-25蛋白表达的影响被引量：1

Effect of hypothyroidism on the SNAP-25 expression in the prefrontal lobe of adult rats

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摘要目的研究成年期甲状腺功能减退症(甲减)大鼠额叶内突触相关蛋白SNAP-25的表达,探讨甲减脑损伤的分子机制。方法用丙基硫氧嘧啶(PTU)建立成年期大鼠甲减模型,采用放射免疫法测定甲减组和正常对照组大鼠的血清甲状腺素水平,免疫组化方法分析两组大鼠额叶内SNAP-25蛋白的表达情况。结果甲减组大鼠血清T3、T4水平显著低于正常对照组(P<0.01、P<0.05);额叶Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ层SNAP-25蛋白的表达水平显著高于正常对照组(P<0.05、P<0.01)。结论成年期甲减大鼠额叶SNAP-25蛋白表达增多。 Objective To observe the expression of synaptosome-associated protein of 25 ku （SNAP-25） in prefrontal lobe of adult-onset hypothyroidism rats and to explore the molecular mechanism of brain.Methods All 30 adult male Sprague-Dawley rats were separated into 2 groups randomly：Hypothyroidism group（n=15） and control normal group（n=15）.The hypothyroidism models were replicated with 6-n-propyl-2-thiouracil（PTU）.The levels of serum T3,T4 were assayed by radioimmunoassay method,and the level of SNAP-25 in prefrontal lobe was analyzed by immunohistochemistry.Results In the hypothyroidism group,the levels of serum T3 and T4 were significantly lower than those in the normal control group（P〈0.05）;and the levels of SNAP-25 protein in theⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ layers of prefrontal lobe were significantly higher than the normal control group（P〈0.05）.Conclusion The expression of SNAP-25 in the prefrontal lobe of adult-onset hypothyroidism can be increased.

作者杨会云王茜孙春萍桂丽朱德发

机构地区安徽医科大学第一附属医院老年内分泌科安徽医科大学第三附属医院内分泌科安徽医科大学综合实验室

出处《安徽医科大学学报》 CAS 北大核心 2010年第4期471-474,共4页 Acta Universitatis Medicinalis Anhui

基金国家自然科学基金(编号:30772301) 安徽省自然科学基金(编号:070413084)

关键词甲状腺功能减退症额叶免疫组织化学突触锥体细胞 hypothyroidism frontal lobe immunohistochemistry synapses pyramidal cells

分类号 R581.2 [医药卫生—内分泌] R322.81 [医药卫生—人体解剖和组织胚胎学]

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