摘要
目的 构建谷胱甘肽S转移酶 -血管生成抑制素 (GST -Angiostatin)融合蛋白表达载体。方法 把血管生成抑制素 (Angiostatin)基因插入融合基因表达载体pGEX - 2T的多克隆位点上 ,转化大肠杆菌DH5α ,提取质粒DNA ,限制性内切酶消化DNA ,琼脂糖凝胶电泳。结果 经限制性内切酶EcoRⅠ、BamHⅠ、PstⅠ酶切质粒DNA ,电泳结果证明Angiostatin基因已插入到pGEX - 2T中。结论 成功构建GST -Angiostatin融合蛋白表达载体 ,为获得较大量的基因工程Angiostatin产品 ,为肿瘤治疗研究作必要准备。
Objective Construction of expression vector of GST-Angiostatin fusion protein.Methods The Angiostatin gene was inserted into the MCS of a fusion gene vector pGEX-2T,the recombinant vector was transformed into E.coli DH5α,extract plasmid DNA,digest plasmid DNA with restriction endonuclease and electrophoresis.Results The recombinant plasmid DNA was digested with restriction endonuclease EcoR Ⅰ,BamH Ⅰ and Pst Ⅰ,the result demonstrates that the Angiostatin gene was successfully inserted into pGEX-2T.Conclusion Successfully constructing expression vector of GST-Angiostatin fusion protein,can provide an essential preparation for obtaining a larger quantity of recombinant human Angiostatin to study it's anti-tumor effects.
出处
《广东医学》
CAS
CSCD
1999年第2期83-84,共2页
Guangdong Medical Journal
